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Titolo:
Expression and role of matrix metalloproteinases MMP-2 and MMP-9 in human spinal column tumors
Autore:
Gokaslan, ZL; Chintala, SK; York, JE; Boyapati, V; Jasti, S; Sawaya, R; Fuller, G; Wildrick, DM; Nicolson, GL; Rao, JS;
Indirizzi:
Univ Texas, MD Anderson Cancer Ctr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas, MD Anderson Cancer Ctr, Dept Pathol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 r Ctr, Dept Pathol, Houston, TX 77030 USA Inst Mol Med, Huntington Beach, CA 92649 USA Inst Mol Med Huntington Beach CA USA 92649 Huntington Beach, CA 92649 USA
Titolo Testata:
CLINICAL & EXPERIMENTAL METASTASIS
fascicolo: 8, volume: 16, anno: 1998,
pagine: 721 - 728
SICI:
0262-0898(199811)16:8<721:EAROMM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GIANT-CELL TUMOR; TISSUE INHIBITOR; IV COLLAGENASES; STROMAL CELLS; COLLAGENOLYTIC ACTIVITY; MESSENGER-RNA; CANCER; METASTASIS; BONE; PHENOTYPE;
Keywords:
immunohistochemistry; MMPs; proteases; spinal metastases; TIMPs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Gokaslan, ZL Univvd,xas, MD Anderson Cancer Ctr, Dept Neurosurg, Box 64,1515 Holcombe Bl Univ Texas Box 64,1515 Holcombe Blvd Houston TX USA 77030 Bl
Citazione:
Z.L. Gokaslan et al., "Expression and role of matrix metalloproteinases MMP-2 and MMP-9 in human spinal column tumors", CLIN EXP M, 16(8), 1998, pp. 721-728

Abstract

Matrix metalloproteinases (MMPs) have been implicated in the process of tumor invasion and metastasis formation, Thus, we determined the expression of MMPs in various primary and metastatic spinal tumors in order to assess the role of these enzymes in spinal invasion, MMP expression was examined byimmunohistochemical localization, and quantitative evaluation of MMP protein content was determined by enzyme-linked immunosorbant assay (ELISA) and Western blotting. MMP enzyme activity was determined by gelatin zymography. Lung carcinomas and melanomas metastatic to the spine were shown to have higher levels of MMP-9 activity than those of breast, thyroid, renal metastases and primary spinal tumors. Immunohistochemical analysis revealed similar difference in expression of MMP-9 in tissue samples, When the tissue samples were subjected to gelatin zymography for examination of MMP-2 and MMP-9activity and to ELISA and Western blotting for quantitative estimation of protein content, the most striking results were obtained for lung carcinomas and melanomas relative to the other tumors. Lung carcinomas and melanomasmetastatic to the spine had considerably higher levels of MMP-9 activity than those of primary spinal tumor or breast, thyroid, acid renal carcinoma metastases, Within the metastatic tumor category, neoplasms that are known to be associated with the shortest overall survival rates and most aggressive behavior, such as lung carcinomas and melanomas, had the highest levels of MMP-2 and MMP-9 activity compared to those less aggressive metastatic tumors such as breast, renal cell, and thyroid carcinomas, Our results suggest that MMPs may contribute to the metastases to the spinal column, and overexpression of these enzymes may correlate with enhanced invasive propertiesof both primary and metastatic spinal tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 00:22:30