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Titolo:
Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae)
Autore:
Itaya, NM; Figueiredo-Ribeiro, RCL; Buckeridge, MS;
Indirizzi:
Secretaria Meio Ambiente Estado Sao Paulo, Inst Bot, Secao Fisiol & Bioquim Secretaria Meio Ambiente Estado Sao Paulo Sao Paulo Brazil BR-01061970 BC
Titolo Testata:
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
fascicolo: 4, volume: 32, anno: 1999,
pagine: 435 - 442
SICI:
0100-879X(199904)32:4<435:SOFBFF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CICHORIUM-INTYBUS L; DE-NOVO SYNTHESIS; HELIANTHUS-TUBEROSUS; IN-VITRO; LOLIUM-TEMULENTUM; EXCISED LEAVES; BIOSYNTHESIS; ENZYMES; PURIFICATION; INULIN;
Keywords:
inulin; fructosyltransferase; fructan biosynthesis; Viguiera discolor; Asteraceae;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Figueiredo-Ribeiro, RCL Secretaria Meio Ambiente Estado Sao Paulo, Inst Bot, Secao Fisiol & Bioquim Secretaria Meio Ambiente Estado Sao Paulo Caixa Postal 4005 Sao Paulo Brazil BR-01061970 BC
Citazione:
N.M. Itaya et al., "Synthesis of fructans by fructosyltransferase from the tuberous roots of Viguiera discolor (Asteraceae)", BRAZ J MED, 32(4), 1999, pp. 435-442

Abstract

Sucrose:sucrose fructosyltransferase (SST) and fructan:fructan fructosyl-transferase (FFT) activities from crude extracts of tuberous roots of Viguiera discolor growing in a preserved area of cerrado were analyzed in 1995-1996. SST activity was characterized by the synthesis of 1-kestose from sucrose and FFT activity by the production of nystose from 1-kestose. The highest fructan-synthesizing activity was observed during early dormancy (autumn), when both (SST and FFT) activities were high. The increase in synthetic activity seemed to start during the fruiting phase in the summer, when SST activity was higher than in spring. During winter and at the beginning of sprouting, both activities declined. The in vitro synthesis of high molecularmass fructans from sucrose by enzymatic preparations from tuberous roots collected in summer showed that long incubations of up to 288 h produced consistently longer polymers which resembled those found in vivo with respect to chromatographic profiles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:43:31