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Titolo:
Determination by MAD-DM of the structure of the DNA duplex d[ACGTACG(5-BrU)](2) at 1.46 angstrom and 100 K
Autore:
Todd, AK; Adams, A; Powell, HR; Wilcock, DJ; Thorpe, LF; Lausi, A; Zanini, F; Wakelin, LPG; Cardin, CJ;
Indirizzi:
Univ Reading, Dept Chem, Reading RG6 6AD, Berks, England Univ Reading Reading Berks England RG6 6AD eading RG6 6AD, Berks, England Univ Dublin Trinity Coll, Dept Biochem, Dublin 2, Ireland Univ Dublin Trinity Coll Dublin Ireland 2 ept Biochem, Dublin 2, Ireland Univ Cambridge, Chem Lab, Cambridge CB2 1EW, England Univ Cambridge Cambridge England CB2 1EW Lab, Cambridge CB2 1EW, England Sincrotrone Elettra, I-34012 Trieste, Italy Sincrotrone Elettra Trieste Italy I-34012 lettra, I-34012 Trieste, Italy Natl Univ Ireland Univ Coll Dublin, Dept Chem, Dublin 4, Ireland Natl UnivIreland Univ Coll Dublin Dublin Ireland 4 m, Dublin 4, Ireland
Titolo Testata:
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
, volume: 55, anno: 1999,
parte:, 4
pagine: 729 - 735
SICI:
0907-4449(199904)55:<729:DBMOTS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIWAVELENGTH ANOMALOUS DIFFRACTION; NUCLEIC-ACIDS; ISOMORPHOUS REPLACEMENT; GEOMETRIC PARAMETERS; GROOVES; CRYSTAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Cardin, CJ Univ Reading, Dept Chem, Reading RG6 6AD, Berks, England Univ Reading Reading Berks England RG6 6AD 6AD, Berks, England
Citazione:
A.K. Todd et al., "Determination by MAD-DM of the structure of the DNA duplex d[ACGTACG(5-BrU)](2) at 1.46 angstrom and 100 K", ACT CRYST D, 55, 1999, pp. 729-735

Abstract

A four-wavelength MAD experiment on a new brominated octanucleotide is reported here. d[ACGTACG(5-BrU)], C77H81BTN30O32P7, M-r, (DNA) = 2235, tetragonal, P4(3)2(1)2 (No. 96), a = 43.597, c = 26.268 Angstrom, V = 49927.5 Angstrom(3), Z = 8, T = 100 K, R = 10.91% for 4312 reflections between 15.0 and1.46 Angstrom resolution. The self-complementary brominated octanucleotided[ACGTACG(S-BrU)](2) has been crystallized and data measured to 1.45 Angstrom at both 293 K and a second crystal hash frozen at 100 K. The latter data collection was carried out to the same resolution at the four wavelengths0.9344, 0.9216, 0.9208 and 0.9003 Angstrom, around the Br K edge at 0.92 Angstrom and the structure determined from a map derived from a MAD data analysis using pseudo-MIR methodology, as implemented in the program MLPHARE. This is one of the first successful MAD phasing experiments carried out at Sincrotrone Elettra in Trieste, Italy. The structure was refined using the data measured at 0.9003 Angstrom, anisotropic temperature factors and the restrained least-squares refinement implemented in the program SHELX96, and the helical parameters are compared with those previously determined for the isomorphous d(ACGTACGT)(2) analogue. The asymmetric unit consists of a single strand of octamer with 96 water molecules. No countercations were located. The A-DNA helix geometry obtained has been analysed using the CURVES program.

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Documento generato il 29/11/20 alle ore 02:44:00