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Titolo:
Bicoid functions without its TATA-binding protein-associated factor interaction domains
Autore:
Schaeffer, V; Janody, F; Loss, C; Desplan, C; Wimmer, EA;
Indirizzi:
Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10021 USA Rockefeller Univ New York NY USA 10021 s Med Inst, New York, NY 10021 USA Inst Biol Dev Marseille, F-13288 Marseille 09, France Inst Biol Dev Marseille Marseille France 09 F-13288 Marseille 09, France
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 8, volume: 96, anno: 1999,
pagine: 4461 - 4466
SICI:
0027-8424(19990413)96:8<4461:BFWITP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEGMENTATION GENE HUNCHBACK; EARLY DROSOPHILA EMBRYO; TRANSCRIPTIONAL ACTIVATORS; COACTIVATOR REQUIREMENTS; ANTERIOR PATTERN; DOWN-REGULATION; BODY PATTERN; RNA-BINDING; EXPRESSION; YEAST;
Keywords:
hunchback; morphogen; TAF(II); transcription;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Desplan, C Rockefeller Univ, Howard Hughes Med Inst, Box 151, New York, NY10021 USA Rockefeller Univ Box 151 New York NY USA 10021 rk, NY 10021 USA
Citazione:
V. Schaeffer et al., "Bicoid functions without its TATA-binding protein-associated factor interaction domains", P NAS US, 96(8), 1999, pp. 4461-4466

Abstract

Four maternal systems are known to pattern the early Drosophila embryo. The key component of the anterior system is the homeodomain protein Bicoid (Bcd). Bcd needs the contribution of another anterior morphogen, Hunchback (Hb), to function properly: Bcd and Hb synergize to organize anterior development. A molecular mechanism for this synergy has been proposed to involve specific interactions of Bcd and Hb with TATA-binding protein-associated factors (TAF(II)s) that are components of the general transcription machinery. Bcd contains three putative activation domains: a glutamine-rich region, which interacts in vitro with TAF(II)110; an alanine-rich domain, which targets TAF(II)60; and a C-terminal acidic region, which has an unknown role. We have generated flies carrying bcd transgenes lacking one or several of these domains to test their function in vivo. Surprisingly, a bcd transgene that lacks all three putative activation domains is able to rescue the bcd(E1) null phenotype to viability. Moreover, the development of these embryos is not affected by the presence of dominant negative mutations in TAF(II)110 or TAF(II)60. This means that the interactions observed in vitro between Bcd and TAF(II)60 or TAF(II)110 aid transcriptional activation but are dispensable for normal development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:37:40