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Titolo:
Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca)
Autore:
Mbeguie-A-Mbeguie, D; Chahine, H; Gomez, RM; Gouble, B; Reich, M; Audergon, JM; Souty, M; Albagnac, G; Fils-Lycaon, B;
Indirizzi:
INRA, Stn Technol Prod Vegetaux, F-84914 Avignon 9, France INRA Avignon France 9 n Technol Prod Vegetaux, F-84914 Avignon 9, France INRA, Stn Rech Fruitieres Mediterraneennes, F-84914 Avignon, France INRA Avignon France F-84914 es Mediterraneennes, F-84914 Avignon, France
Titolo Testata:
PHYSIOLOGIA PLANTARUM
fascicolo: 2, volume: 105, anno: 1999,
pagine: 294 - 303
SICI:
0031-9317(199902)105:2<294:MCAEOA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETHYLENE-FORMING ENZYME; SITE-DIRECTED MUTAGENESIS; CUCUMIS-MELO L; HIGHER-PLANTS; ACID OXIDASE; APPLE FRUIT; PROTEIN; BIOSYNTHESIS; GENE; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Fils-Lycaon, B INRA,gnon Technol Prod Vegetaux, Site Agroparc,Domaine St Paul, F-84914 Avi INRA Site Agroparc,Domaine St Paul Avignon France 9 14 Avi
Citazione:
D. Mbeguie-A-Mbeguie et al., "Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca)", PHYSL PLANT, 105(2), 1999, pp. 294-303

Abstract

Little is known on gene expression during ripening of apricot fruit (Prunus armeniaca cv. Bergeron), in particular for the enzymes involved in ethylene biosynthesis. ACC oxidase (EC 1.4.3) catalyses the last step of the ethylene biosynthesis pathway in higher plants. Based on sequence conservation of ACC oxidases, a reverse transcriptase-polymerase chain reaction (RT-PCR)experiment was carried out to synthesize a homologous ACC oxidase probe from apricot fruit. This probe was further used to isolate a full length ACC oxidase cDNA, Pa-ACO, from a ripe fruit cDNA library. Pa-ACO is 1 236 bp long and contains a single open reading frame encoding a mature peptide of 319 amino acids with a calculated molecular mass of 36.17 kDa and a pI of 5.07. Immunoblot analysis allowed us to associate a 40 kDa protein to apricot fruit ACC oxidase. Pa-ACO belongs to a multi-gene family, is fruit specificand is transcriptionally regulated during ripening of apricot fruit.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:34:44