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Titolo:
Caspase-mediated cleavage of cytoskeletal actin plays a positive role in the process of morphological apoptosis
Autore:
Mashima, T; Naito, M; Tsuruo, T;
Indirizzi:
Univ3,okyo, Inst Mol & Cellular Biosci, Biomed Res Lab, Bunkyo Ku, Tokyo 11 Univ Tokyo Tokyo Japan 113 r Biosci, Biomed Res Lab, Bunkyo Ku, Tokyo 11 Japanese Fdn Canc Res, Ctr Canc Chemotherapy, Tokyo 170, Japan Japanese Fdn Canc Res Tokyo Japan 170 anc Chemotherapy, Tokyo 170, Japan
Titolo Testata:
ONCOGENE
fascicolo: 15, volume: 18, anno: 1999,
pagine: 2423 - 2430
SICI:
0950-9232(19990415)18:15<2423:CCOCAP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERLEUKIN 1-BETA-CONVERTING ENZYME; AGENT-INDUCED APOPTOSIS; ICE-LIKE PROTEASES; INTERLEUKIN-1-BETA-CONVERTING ENZYME; CELL-DEATH; CYTOCHROME-C; PHOSPHATIDYLSERINE EXTERNALIZATION; DNA FRAGMENTATION; ACTIVATION; GELSOLIN;
Keywords:
caspase; actin; etoposide; morphology; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuruo, T Univyoi,yo, Inst Mol & Cellular Biosci, Biomed Res Lab, Bunkyo Ku, 1-1-1 Ya Univ Tokyo 1-1-1 Yayoi Tokyo Japan 113 Lab, Bunkyo Ku, 1-1-1 Ya
Citazione:
T. Mashima et al., "Caspase-mediated cleavage of cytoskeletal actin plays a positive role in the process of morphological apoptosis", ONCOGENE, 18(15), 1999, pp. 2423-2430

Abstract

Tumors result from the imbalance between cell growth and apoptosis. One ofthe characteristic changes in cancers is the abnormality in cytoskeleton, which suggests some roles of cytoskeletal proteins in tumorigenesis or the maintenance of tumor cells. Previously we showed that cytoskeletal actin isthe substrate of caspases, the proteases responsible for apoptosis, while the role of actin cleavage in apoptosis remained unknown. To examine the cleavage of actin in vivo, we extensively performed immunoblot analysis usingactin fragment-specific antibody. Here, we showed that, in some solid tumor cells, induction of apoptosis was accompanied by caspase-dependent actin-cleavage to 15 and 31 kDa fragments in vivo. To elucidate the role of actin-cleavage further, we introduced actin cleaved-fragments. We found that ectopic expression of an actin 15 kDa fragment induces morphological changes resembling those of apoptotic cells, The expression of the actin fragment induced a dramatic change of cellular actin localization, as visualized by enhanced green fluorescent protein (EGFP)-tagged actin, while the actin fragment expression did not cause caspase activation nor the cleavage of a marker substrate protein, poly (ADP-ribose) polymerase. These results indicate that actin cleavage could play a positive role in the morphological changes of apoptosis downstream of caspase activation.

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Documento generato il 04/12/20 alle ore 22:05:39