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Titolo:
Regulation of brain glucose transporters by glucose and oxygen deprivation
Autore:
Bruckner, BA; Ammini, CV; Otal, MP; Raizada, MK; Stacpoole, PW;
Indirizzi:
Univ2610rida, Coll Med, Dept Med, Div Endocrinol & Metab, Gainesville, FL 3 Univ Florida Gainesville FL USA 32610 docrinol & Metab, Gainesville, FL 3 Univ Florida, Coll Med, Dept Physiol, Gainesville, FL 32610 USA Univ Florida Gainesville FL USA 32610 Physiol, Gainesville, FL 32610 USA Univ Florida, Coll Med, Dept Biochem & Mol Biol, Gainesville, FL 32610 USAUniv Florida Gainesville FL USA 32610 Mol Biol, Gainesville, FL 32610 USA
Titolo Testata:
METABOLISM-CLINICAL AND EXPERIMENTAL
fascicolo: 4, volume: 48, anno: 1999,
pagine: 422 - 431
SICI:
0026-0495(199904)48:4<422:ROBGTB>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUT1 MESSENGER-RNA; GENE-EXPRESSION; RAT-BRAIN; 3T3-L1 ADIPOCYTES; GLIAL-CELLS; OXIDATIVE-PHOSPHORYLATION; DEVELOPMENTAL REGULATION; PROTEIN-SYNTHESIS; PRIMARY CULTURES; ANGIOTENSIN-II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Stacpoole, PW Univsville,a, Coll Med, Dept Med, Div Endocrinol & Metab, Box 100226, Gaine Univ Florida Box 100226 Gainesville FL USA 32610 0226, Gaine
Citazione:
B.A. Bruckner et al., "Regulation of brain glucose transporters by glucose and oxygen deprivation", METABOLISM, 48(4), 1999, pp. 422-431

Abstract

Brain cells are dependent on glucose and oxygen for energy, We investigated the effects of hypoxia, glucose deprivation, and hypoxia plus glucose deprivation on mRNA and protein levels of glucose transporter (GLUT1) and GLUT3 and 2-deoxyglucose (2-DG) uptake in primary cultures of rat neurons and astroglia, Hypoxia for 24 hours did not significantly affect cell viability but increased neuronal GLUT1 and GLUT3 mRNA up to 40-fold and fivefold, respectively, above control levels, Similar changes in GLUT1 mRNA were measured in glia, The effects of hypoxia on GLUT1 and GLUT3 mRNA were reversible, The increase in GLUT1 mRNA could be detected within 20 minutes of hypoxia and was blocked by actinomycin D. Nuclear runoff transcription assays showedthat hypoxia did not alter the transcription rate of GLUT1, However, hypoxia enhanced the stability of GLUT1 mRNA in neurons (half-life [t(1/2)] > 12hours) compared with normoxic conditions (t(1/2) similar to 10.4 hours), suggesting the existence of a posttranscriptional mechanism far the regulation of GLUT1 transcript levels, Twenty-four hours of normoxia and 1.0 mmoI/Lglucose increased neuronal GLUT1 mRNA less than threefold above basal, but24 hours of glucose and oxygen deprivation increased GLUT1 over 111-fold above basal. Induction of neuronal GLUT1 mRNA was temporally associated withincreased levels of GLUT1 protein and with stimulation of intracellular 2-DG accumulation. We conclude that hypoxia reversibly increases the transcript revels of GLUT1 and GLUT3 in rat brain cells and stimulates GLUT1 transcript levels by posttranscriptional mechanisms. Although glucose deprivatianalone produces minimal effects on GLUT mRNA levels, hypoxia plus glucose deprivation synergize to markedly increase GLUT gene expression. Copyright (C) 1999 by W.B. Saunders Company.

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Documento generato il 06/07/20 alle ore 08:23:46