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Titolo:
LOCALIZATION OF UROKINASE TO FOCAL ADHESIONS BY HUMAN FIBROSARCOMA CELLS SYNTHESIZING RECOMBINANT VITRONECTIN
Autore:
WILCOX SA; REHO T; HIGGINS PJ; TOMINNASEBALD E; MCKEOWNLONGO PJ;
Indirizzi:
ALBANY MED COLL UNION UNIV,DEPT PHYSIOL & CELL BIOL,ALBANY MED COLL,47 NEW SCOTLAND AVE ALBANY NY 12208 ALBANY MED COLL UNION UNIV,DEPT PHYSIOL & CELL BIOL,ALBANY MED COLL ALBANY NY 12208 ALBANY MED COLL UNION UNIV,DEPT PHYSIOL & CELL BIOL,DEPT MICROBIOL MOL GENET & IMMUNOL ALBANY NY 12208
Titolo Testata:
Biochemistry and cell biology
fascicolo: 6, volume: 74, anno: 1996,
pagine: 899 - 910
SICI:
0829-8211(1996)74:6<899:LOUTFA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR INHIBITOR; RECEPTOR-BOUND UROKINASE; SOMATOMEDIN-B DOMAIN; ENDOTHELIAL-CELLS; GENE-EXPRESSION; HUMAN-MELANOMA; SURFACE UROKINASE; HUMAN-MONOCYTES; ALPHA-THROMBIN; HT-1080 CELLS;
Keywords:
UROKINASE; VITRONECTIN; FOCAL ADHESIONS; ALPHA(V)BETA(5) INTEGRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
S.A. Wilcox et al., "LOCALIZATION OF UROKINASE TO FOCAL ADHESIONS BY HUMAN FIBROSARCOMA CELLS SYNTHESIZING RECOMBINANT VITRONECTIN", Biochemistry and cell biology, 74(6), 1996, pp. 899-910

Abstract

Cell surface plasminogen activators have been proposed to participatein cell migration and invasion by activating both intracellular signaling pathways and extracellular proteolysis. Urokinase-type plasminogen activator (uPA) is secreted from many cell types and localizes to focal contact areas when cells are seeded onto the plasma protein vitronectin. Induction of vitronectin synthesis during migration of neural crest cells and growth of certain tumors suggests that the de novo synthesis and deposition of vitronectin into the tissue matrix may remodelthe matrix to provide an environment suitable for cell migration and (or) tumor invasion. To investigate the effects of vitronectin secretion and matrix deposition on the localization and activity of cell-associated uPA, HT-1080 fibrosarcoma cells were transfected with the Rc/CMV expression vector containing a vitronectin cDNA insert and stable cell lines expressing vitronectin were selected. Vitronectin-secreting cells were allowed to attach and spread on collagen- and fibronectin-coated substrates. Within 6 h, vitronectin was detected on the substrate; vitronectin synthesis was accompanied by the clustering of both the alpha(v) beta(5) vitronectin receptor and uPA into vinculin-containingfocal adhesions. Although mock transfected cells formed small focal adhesions on both collagen and fibronectin, no co-localization of uPA or alpha(v) beta(5) to focal adhesions was evident in these cells. Vitronectin-secreting cells also exhibited decreased levels of plasminogenactivation and increased levels of cell adhesion as compared with themock transfected cells. These data demonstrate that the synthesis of vitronectin and its matrix association by transfected HT-1080 fibrosarcoma cells results in localization of uPA to alpha(v) beta(5) containing focal adhesions, decreased cell surface uPA activity, and an increase in cell adhesion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:18:43