Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Influence of sample preparation technique on two-dimensional gel electrophoresis of proteins from Porphyromonas gingivalis
Autore:
Pridmore, AM; Devine, DA; Bonass, WA; Silley, P;
Indirizzi:
Univlandds, Leeds Dent Inst, Div Oral Biol, Leeds LS2 9JT, W Yorkshire, Eng Univ Leeds Leeds W Yorkshire England LS2 9JT ds LS2 9JT, W Yorkshire, Eng Don Whitley Sci Ltd, Shipley, England Don Whitley Sci Ltd Shipley England n Whitley Sci Ltd, Shipley, England
Titolo Testata:
LETTERS IN APPLIED MICROBIOLOGY
fascicolo: 4, volume: 28, anno: 1999,
pagine: 245 - 249
SICI:
0266-8254(199904)28:4<245:IOSPTO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
2-DIMENSIONAL ELECTROPHORESIS; ESCHERICHIA-COLI; STRESS-RESPONSE; W50; ENZYMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Pridmore, AM Univlandds, Leeds Dent Inst, Div Oral Biol, Leeds LS2 9JT, W Yorkshire, Eng Univ Leeds Leeds W Yorkshire England LS2 9JT Yorkshire, Eng
Citazione:
A.M. Pridmore et al., "Influence of sample preparation technique on two-dimensional gel electrophoresis of proteins from Porphyromonas gingivalis", LETT APPL M, 28(4), 1999, pp. 245-249

Abstract

Sample preparation methods were compared for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of cellular proteins from the proteolytic bacterium Porphyromonas gingivalis. Standard solubilization buffer yielded poorly resolved protein spots, but pre-treatment of cells with trichloroacetic acid or inclusion of the protease inhibitor TLCK during solubilization improved definition and separation. The latter approach allowed reliable detection of a 55 kDa immunodominant surface antigen by Western immunoblotting. Further improvements in resolution occurred when SDS was included in the sample preparation. Thus, controlling proteolysis and optimizing protein solubilization were essential for reproducible separations and maximal protein recovery during 2D-PAGE of P. gingivalis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:55:15