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Titolo:
Polyvalent Rev decoys act as artificial Rev-responsive elements
Autore:
Symensma, TL; Baskerville, S; Yan, A; Ellington, AD;
Indirizzi:
Univ Texas, Dept Chem, Austin, TX 78712 USA Univ Texas Austin TX USA 78712 niv Texas, Dept Chem, Austin, TX 78712 USA Indiana Univ, Dept Microbiol, Bloomington, IN 47405 USA Indiana Univ Bloomington IN USA 47405 icrobiol, Bloomington, IN 47405 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 5, volume: 73, anno: 1999,
pagine: 4341 - 4349
SICI:
0022-538X(199905)73:5<4341:PRDAAA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; GENE-EXPRESSION REQUIRES; NUCLEAR EXPORT SIGNALS; VIRAL MESSENGER-RNA; HIV-1 REV; TYPE-1 REV; ACTIVATION DOMAIN; TRANS-ACTIVATOR; TARGET SEQUENCE; BINDING-SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Ellington, AD Univ Texas, Dept Chem, ICMB A4800, Austin, TX 78712 USA UnivTexas ICMB A4800 Austin TX USA 78712 stin, TX 78712 USA
Citazione:
T.L. Symensma et al., "Polyvalent Rev decoys act as artificial Rev-responsive elements", J VIROLOGY, 73(5), 1999, pp. 4341-4349

Abstract

Interactions between Rev and the Rev-responsive element (RRE) control the order, rate, and extent of gene expression in human immunodeficiency virus type 1, Rev decoys may therefore prove to be useful RNA therapeutics for the treatment of AIDS. To improve upon the current generation of Rev decoys that bind single Rev molecules, it would be useful to generate polyvalent Rev decoys that could bind multiple Rev molecules. J, Kjems and P, A, Sharp (J. Virol, 67:4769-4776, 1993) originally constructed functional polyvalent Rev decoys, but the structural context of these polyvalent decoys remains unclear, and it has been argued that the individual decoys were either structurally discrete (Kjems and Sharp, J, Virol, 67:4769-4776, 1993) or were part of an extended helix (R, W, Zemmel et al,, Mel. Biol, 258:763-777, 1996), To resolve the differences between these models, we have designed and synthesized concatemers of Rev-binding elements (RBEs) that fold to form multiple, discrete, high-affinity Rev-binding sites. We find that the concatenated RBEs can facilitate the cytoplasmic transport of viral mRNAs and therefore likely bind multiple Rev molecules, These artificial RREs may simultaneously sequester Rev and hinder access to the cellular transport machinery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 05:29:51