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Titolo:
Optical mapping of neural network activity in chick spinal cord at an intermediate stage of embryonic development
Autore:
Arai, Y; Momose-Sato, Y; Sato, K; Kamino, K;
Indirizzi:
Tokyoaned & Dent Univ, Sch Med, Dept Physiol, Bunkyo Ku, Tokyo 1138519, Jap Tokyo Med & Dent Univ Tokyo Japan 1138519 Bunkyo Ku, Tokyo 1138519, Jap
Titolo Testata:
JOURNAL OF NEUROPHYSIOLOGY
fascicolo: 4, volume: 81, anno: 1999,
pagine: 1889 - 1902
SICI:
0022-3077(199904)81:4<1889:OMONNA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
VOLTAGE-SENSITIVE DYES; BRAIN-STEM; ELECTRICAL-ACTIVITY; VAGAL-STIMULATION; NERVE-TERMINALS; FUNCTIONAL-ORGANIZATION; POTENTIOMETRIC PROBES; NEURONAL-ACTIVITY; RECORDING SYSTEM; RESPONSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kamino, K Tokyokyod & Dent Univ, Sch Med, Dept Physiol, Bunkyo Ku, 1-5-45 Yushima, To Tokyo Med & Dent Univ 1-5-45 Yushima Tokyo Japan 1138519 ma, To
Citazione:
Y. Arai et al., "Optical mapping of neural network activity in chick spinal cord at an intermediate stage of embryonic development", J NEUROPHYS, 81(4), 1999, pp. 1889-1902

Abstract

We have applied multiple-site optical recording of transmembrane potentialchanges to recording of neuronal pathway/network activity from embryonic chick spinal cord slice preparations. Spinal cord preparations were dissected from 8-day-old chick embryos at Hamburger-Hamilton stage 33, and transverse slice preparations were prepared with the 13th cervical spinal nerve or with the 2nd or 5th lumbosacral spinal nerve intact. The slice preparationswere stained with a voltage-sensitive merocyanine-rhodanine dye (NK2761). Transmembrane voltage-related optical (dye-absorbance) changes evoked by spinal nerve stimulation with positive square-current pulses using a suction electrode were recorded simultaneously from many loci in the preparation, using a 128- or 1,020-element photodiode array. Optical responses were detected from dorsal and ventral regions corresponding to the posterior (dorsal)and anterior (ventral) gray horns. The optical signals were composed of two components, fast spike-like and slow signals. Ln the dorsal region, the fast spike-like signal was identified as the presynaptic action potential inthe sensory nerve and the slow signal as the postsynaptic potential. In the ventral region, the fast spike-like signal reflects the antidromic actionpotential in motoneurons, and the slow signal is related to the postsynaptic potential evoked in the motoneuron. In preparations in which the ventralroot was cut microsurgically, the antidromic action potential-related optical signals were eliminated. The areas of the maximal amplitude of the evoked signals in the dorsal and ventral regions were located near the dorsal root entry zone and the ventral root outlet zone, respectively. Quasiconcentric contour line maps were obtained in the dorsal and ventral regions, suggesting the functional arrangement of the dorsal and ventral synaptic connections. Synaptic fatigue induced by repetitive stimuli in the ventral synapses was more rapid than in the dorsal synapses. The distribution patterns ofthe signals were essentially similar among C13, LS2, and LS5 preparations,suggesting that there is no difference in the spatiotemporal pattern of the neural responses along the rostrocaudal axis of the spinal cord at this developmental stage. In the ventral root-cut preparations, comparing the delay times between the ventral slow optical signals, we have been able to demonstrate that neural network-related synaptic connections are generated functionally in the embryonic spinal cord at Hamburger-Hamilton stage 33.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:34:30