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Titolo:
Different antigenic reactivities of bovine brain glutamate dehydrogenase isoproteins
Autore:
Choi, SY; Hong, JW; Song, MS; Jeon, SG; Bahn, JH; Lee, BR; Ahn, JY; Cho, SW;
Indirizzi:
Hallym Univ, Dept Genet Engn, Div Life Sci, Chunchon 200702, South Korea Hallym Univ Chunchon South Korea 200702 ci, Chunchon 200702, South Korea Univ Ulsan, Coll Med, Dept Biochem, Seoul, South Korea Univ Ulsan Seoul South Korea Coll Med, Dept Biochem, Seoul, South Korea
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 5, volume: 72, anno: 1999,
pagine: 2162 - 2169
SICI:
0022-3042(199905)72:5<2162:DAROBB>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEGENERATIVE NEUROLOGICAL DISORDERS; RNA-BINDING PROTEIN; MEMBRANE-BOUND FORM; INSULIN RELEASE; GENE FAMILY; IDENTIFICATION; SITE; LIVER; HYPERAMMONEMIA; ANTIBODIES;
Keywords:
glutamate dehydrogenase isoproteins; monoclonal antibodies; protein-protein interaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Choi, SY Hallym2,niv, Dept Genet Engn, Div Life Sci, 1-1 Okchon-Dong, Chunchon 20070 Hallym Univ 1-1 Okchon-Dong Chunchon South Korea 200702 on 20070
Citazione:
S.Y. Choi et al., "Different antigenic reactivities of bovine brain glutamate dehydrogenase isoproteins", J NEUROCHEM, 72(5), 1999, pp. 2162-2169

Abstract

The structural differences between two types of glutamate dehydrogenase (GDH) isoproteins (GDH I and GDH II), homogeneously isolated from bovine brain, were investigated using a biosensor technology and monoclonal antibodies. A total of seven monoclonal antibodies raised against GDH II were produced, and the antibodies recognized a single protein band that comigrates withpurified GDH II on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot, Of seven anti-GDH II monoclonal antibodies tested in theimmunoblot analysis, all seven antibodies interacted with GDH II, whereas only four antibodies recognized the protein band of the other GDH isoprotein, GDH I, When inhibition tests of the GDH isoproteins were performed with the seven anti-GDH II monoclonal antibodies, three antibodies inhibited GDHII activity, whereas only one antibody inhibited GDH I activity. The binding affinity of anti-GDH II monoclonal antibodies for GDH II (K-D = 1.0 nM) determined using a biosensor technology (Pharmacia BIAcore) was fivefold higher than for GDH I (K-D = 5.3 nM), These results, together with epitope mapping analysis, suggest that there may be structural differences between the two GDH isoproteins, in addition to their different biochemical properties. Using the anti-GDH II antibodies as probes, we also investigated the crossreactivities of brain GDHs from some mammalian and an avian species, showing that the mammalian brain GDH enzymes are related immunologically to each other.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:26:47