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Titolo:
Continuous primary sequence requirements in the 18-nucleotide promoter of dicot plant mitochondria
Autore:
Dombrowski, S; Hoffmann, M; Guha, C; Binder, S;
Indirizzi:
Univ Ulm, Allgemeine Bot, D-89069 Ulm, Germany Univ Ulm Ulm Germany D-89069 v Ulm, Allgemeine Bot, D-89069 Ulm, Germany
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 15, volume: 274, anno: 1999,
pagine: 10094 - 10099
SICI:
0021-9258(19990409)274:15<10094:CPSRIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; RNA-POLYMERASES; GENE-EXPRESSION; MESSENGER-RNA; IN-VITRO; IDENTIFICATION; ARABIDOPSIS; DNA; PEA; PLASTIDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Binder, S Univ Ulm, Allgemeine Bot, Albert-Einstein-Allee 11, D-89069 Ulm,Germany Univ Ulm Albert-Einstein-Allee 11 Ulm Germany D-89069 , Germany
Citazione:
S. Dombrowski et al., "Continuous primary sequence requirements in the 18-nucleotide promoter of dicot plant mitochondria", J BIOL CHEM, 274(15), 1999, pp. 10094-10099

Abstract

The nucleotide requirements of mitochondrial promoters of dicot plants were studied in detail in a pea in vitro transcription system. Deletions in the 5' regions of three different transcription initiation sites from pea, soybean, and Oenothera identified a crucial AT-rich sequence element (AT-Box)comprising nucleotide positions -14 to -9 relative to the first transcribed nucleotide. Transversion of the AT-Box sequence to complementary nucleotide identities results in an almost complete loss of promoter activity, suggesting that primary structure rather than a simple accumulation of adeninesand thymidines in this region is essential for promoter activity. This promoter segment thus appears to be involved in sequence specific binding of arespective protein factor(s) rather than merely loosening and melting the DNA helix during or for an initiation event. Manipulation of nucleotide identities in the 3' portion of the pea atp9 promoter and the respective S'-flanking region revealed that essential sequences extend to positions +3/+4 beyond this transcription start site. Efficient transcription initiation at an 18-base pair promoter sequence ranging from nucleotide positions -14 to 4 integrated into different sequence contexts shows this element to be sufficient for autonomous promoter function independent of surrounding sequences.

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Documento generato il 23/09/20 alle ore 15:34:11