Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Expression of genes involved in calcium absorption in human duodenum
Autore:
Walters, JRF; Howard, A; Lowery, LJ; Mawer, EB; Legon, S;
Indirizzi:
HammersmithN,osp, Imperial Coll, Sch Med, Gastroenterol Unit, London W12 0N Hammersmith Hosp London England W12 0NN astroenterol Unit, London W12 0N Hammersmithngland Imperial Coll, Sch Med, Dept Metab Med, London W12 0NN, E Hammersmith Hosp London England W12 0NN ept Metab Med, London W12 0NN, E Univ Manchester, Dept Med, Manchester M13 9PL, Lancs, England Univ Manchester Manchester Lancs England M13 9PL M13 9PL, Lancs, England
Titolo Testata:
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
fascicolo: 3, volume: 29, anno: 1999,
pagine: 214 - 219
SICI:
0014-2972(199903)29:3<214:EOGIIC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAMIN-D-RECEPTOR; BONE-MINERAL DENSITY; BINDING PROTEIN; MESSENGER-RNA; CALBINDIN-D; SERUM 1,25-DIHYDROXYVITAMIN-D; POSTMENOPAUSAL WOMEN; DIETARY CALCIUM; SMALL-INTESTINE; RAT INTESTINE;
Keywords:
absorption; calbindin-d9k; Ca2+-ATPase; 1,25-dihydroxyvitamin D; duodenum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Walters, JRF HammersmithN,osp, Imperial Coll, Sch Med, Gastroenterol Unit,London W12 0N Hammersmith Hosp London England W12 0NN Unit, London W12 0N
Citazione:
J.R.F. Walters et al., "Expression of genes involved in calcium absorption in human duodenum", EUR J CL IN, 29(3), 1999, pp. 214-219

Abstract

Background The role of Vitamin D metabolites in the regulation of expression of genes involved in dietary calcium absorption by the human intestine remains poorly understood despite much work in animals. Materials and methods To investigate this, we measured the expression of transcripts for two of these genes, calbindin-D9k and the basolateral membrane calcium pumping ATPase, PMCA1, in duodenal endoscopic biopsies from 40 subjects. Northern blots were hybridized with previously characterized probes, and the signal was quantified by phosphor imaging. These transcript levels were related to circulating vitamin D metabolites and also analysed according to the vitamin D receptor gene TaqI polymorphism determined in DNA from blood. Results Plasma 1,25-dihydroxycholecalciferol correlated significantly withcalbindin-D9k RNA (r = 0.4, P < 0.02) but not with PMCA1. Plasma 25-OH-cholecalciferol was not correlated with transcripts for either gene and, furthermore, the mean levels of these transcripts did not differ significantly when grouped by vitamin D receptor genotype. Conclusion In normal humans, 1,25-dihydroxycholecalciferol has a small butsignificant relationship to duodenal expression of calbindin-D9k, but not to PMCA1 expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:16:50