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Titolo:
A protocol to cryopreserve nodal explants of Antirrhinum microphyllum by encapsulation-dehydration
Autore:
Gonzalez-Benito, ME; Nunez-Moreno, Y; Martin, C;
Indirizzi:
Univ Politecn Madrid, Dept Biol Vegetal, E-28040 Madrid, Spain Univ Politecn Madrid Madrid Spain E-28040 Vegetal, E-28040 Madrid, Spain Univ Politecn Madrid, Escuela Ingn Tecn Agr, E-28040 Madrid, Spain Univ Politecn Madrid Madrid Spain E-28040 ecn Agr, E-28040 Madrid, Spain UnivSpaintecn Madrid, Escuela Tecn Super Ingenieros Agron, E-28040 Madrid,Univ Politecn Madrid Madrid Spain E-28040 enieros Agron, E-28040 Madrid,
Titolo Testata:
CRYO-LETTERS
fascicolo: 4, volume: 19, anno: 1998,
pagine: 225 - 230
SICI:
0143-2044(199807/08)19:4<225:APTCNE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
COATED SOMATIC EMBRYOS; DAUCUS-CAROTA L; SHOOT-TIPS; LIQUID-NITROGEN; VITRIFICATION; DESICCATION; RESISTANCE; MERISTEMS;
Keywords:
Antirrhinum; cryopreservation; dehydration; encapsulation; endemic; node;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Gonzalez-Benito, ME Univ Politecn Madrid, Dept Biol Vegetal, Ciudad Univ, E-28040 Madrid, Spain Univ Politecn Madrid Ciudad Univ Madrid Spain E-28040
Citazione:
M.E. Gonzalez-Benito et al., "A protocol to cryopreserve nodal explants of Antirrhinum microphyllum by encapsulation-dehydration", CRYO-LETT, 19(4), 1998, pp. 225-230

Abstract

A basic cryopreservation protocol, by encapsulation-dehydration, of nodal explants of the Spanish endemic plant Antirrhinum microphyllum has been successfully employed: preculture of explants on MS medium for 1 day, encapsulation, culture of beads on 0.75 M sucrose for 19 h, desiccation to 20% moisture content, fast freezing and thawing. The effect of moisture content andpreculture on survival and shoot production after freezing was studied.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:23:00