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Titolo:
Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration
Autore:
Zhao, YH; Wu, YJ; Engelmann, F; Zhou, MD; Zhang, DM; Chen, SY;
Indirizzi:
IPGRI, I-00145 Rome, Italy IPGRI Rome Italy I-00145IPGRI, I-00145 Rome, Italy HebeieoplesAgr & Forestry Sci, Changli Inst Pomol, Changli 066600, Hebei, P Hebei Acad Agr & Forestry Sci Changli Hebei Peoples R China 066600 bei, P CAAS, IPGRI, E Asia Off, Beijing 100081, Peoples R China CAAS Beijing Peoples R China 100081 Off, Beijing 100081, Peoples R China
Titolo Testata:
CRYO-LETTERS
fascicolo: 2, volume: 20, anno: 1999,
pagine: 103 - 108
SICI:
0143-2044(199903/04)20:2<103:COASTB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Keywords:
apple; cryopreservation; encapsulation-dehydration; shoot tips; sucrose preculture; dehydration; freezing procedure; physiological state;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
6
Recensione:
Indirizzi per estratti:
Indirizzo: Engelmann, F IPGRI, Via Sette Chiese 142, I-00145 Rome, Italy IPGRI Via Sette Chiese 142 Rome Italy I-00145 45 Rome, Italy
Citazione:
Y.H. Zhao et al., "Cryopreservation of apple shoot tips by encapsulation-dehydration: Effect of preculture, dehydration and freezing procedure on shoot regeneration", CRYO-LETT, 20(2), 1999, pp. 103-108

Abstract

Shoot tips sampled on in vitro cultured apple plantlets of 6 accessions ofM. domestica and one accession of M. robusta were successfully cryopreserved using the encapsulation-dehydration technique. Shoot tips were excised from plantlets which had been submitted to 3 weeks of cold-acclimation at 5 degrees C, 70 d after their last subculture. After preculture at 5 degrees C in media with progressively increased sucrose concentration (0.1 M,0.3 M and 0.7 M), shoot tips were encapsulated and pregrown in medium with 1.0 M sucrose for I d, dehydrated for 4 h under the air current of the laminar flow cabinet, thus reaching a moisture content of around 30% (fresh weight basis) and directly immersed in liquid nitrogen. The regeneration rate of cryopreserved apices varied between 70 and 90%, depending on the accession. Using apices sampled on plantlets which had been maintained on standard medium without subculture for 6 months, sucrose preculture became unnecessary toachieve regrowth after cryopreservation and the dehydration period was shortened. These experiments showed that the physiological state of the plant material directly affects the results and procedures for cryopreservation of apple shoot tips.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:04:24