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Titolo:
Cryopreservation of cassava in vitro-grown shoot tips cooled to -196 degrees C by vitrification.
Autore:
Charoensub, R; Phansiri, S; Sakai, A; Yongmanitchai, W;
Indirizzi:
Kasetsart Univ, Inst Res & Dev, Sci Equipment Ctr, Bangkok 10900, ThailandKasetsart Univ Bangkok Thailand 10900 pment Ctr, Bangkok 10900, Thailand Hokkaido Univ, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 iv, Sapporo, Hokkaido 060, Japan
Titolo Testata:
CRYO-LETTERS
fascicolo: 2, volume: 20, anno: 1999,
pagine: 89 - 94
SICI:
0143-2044(199903/04)20:2<89:COCIVS>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
WASABI WASABIA-JAPONICA; PLANT-REGENERATION; APICAL MERISTEMS; LIQUID-NITROGEN; CELLS; SINENSIS;
Keywords:
cassava; cryopreservation; vitrification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Charoensub, R Kasetsart Univ, Inst Res & Dev, Sci Equipment Ctr, Bangkok 10900, Thailand Kasetsart Univ Bangkok Thailand 10900 gkok 10900, Thailand
Citazione:
R. Charoensub et al., "Cryopreservation of cassava in vitro-grown shoot tips cooled to -196 degrees C by vitrification.", CRYO-LETT, 20(2), 1999, pp. 89-94

Abstract

We first succeeded in the cryopreservation of cassava by a simple vitrification procedure. Shoot tips of cassava (Manihot esculenta Crantz, cv.CM3281-4) were excised from two-week-old in vitro-grown plantlets and preculturedon solidified MS basal medium supplemented with 0.3 M sucrose for 16 h. The precultured shoot tips were treated with a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25 degrees C and then dehydrated with a vitrification solution (PVS2) for 45 min at 25 degrees C prior to a plunge into LN. Successfully vitrified shoot tips resumed growth within a week and developed shoots without intermediary callus formation. The average rate of normalshoot formation amounted to 75%. Thus, this simple protocol by vitrification appears promising as a practical method for cryopreservation of cassava germplasm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 10:09:59