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Titolo:
Voltage-sensitive dye RH421 increases contractility of cardiac muscle
Autore:
Cheng, Y; Van Wagoner, DR; Mazgalev, TN; Tchou, PJ; Efimov, IR;
Indirizzi:
Cleveland Clin Fdn, Dept Cardiol, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 ardiol, Cleveland, OH 44195 USA
Titolo Testata:
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
fascicolo: 12, volume: 76, anno: 1998,
pagine: 1146 - 1150
SICI:
0008-4212(199812)76:12<1146:VDRICO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENT STYRYL DYES; FIELD STIMULATION; INTACT HEARTS; MEMBRANE; PROBES; REPOLARIZATION; CELLS; WAVES;
Keywords:
voltage-sensitive dye RH421; 2,3-butanedione monoxime; left ventricular pressure; cell shortening; contractility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Efimov, IR Cleveland4195n Fdn, Dept Cardiol, Desk FF1,9500 Euclid Ave, Cleveland, OH 4 Cleveland Clin Fdn Desk FF1,9500 Euclid Ave Cleveland OH USA 44195
Citazione:
Y. Cheng et al., "Voltage-sensitive dye RH421 increases contractility of cardiac muscle", CAN J PHYSL, 76(12), 1998, pp. 1146-1150

Abstract

Voltage-sensitive dyes and imaging techniques have proved to be indispensable tools for use in in vitro electrophysiological studies. To avoid motionartifacts in optical recordings, electromechanical uncouplers such as 2,3-butanedione monoxime (BDM) are required. In this study, we sought to determine whether the voltage-sensitive dye RH421 had an effect on the contractility of heart muscle, either drone or in the presence of BDM. Ventricular contractility was studied in (i) isolated rat myocytes and (ii) Langendorff-perfused rat hearts under control conditions, and during perfusion with RH421 or RH421 + BDM. The following results were obtained. (i) The amplitude ofcell shortening increased progressively from 6.24 +/- 0.64 to 9.95 +/- 1.02 mu m during 15 min of superfusion with 5 mu M RH421 (n = 11), and furtherincreased to 12.54 +/- 0.97 mu m during washout. In seven cells first perfused with 15 mM BDM and then with 15 mM EDM + 5 mu M RH421, the amplitude of the cell shortening first decreased from 5.17 +/- 0.51 to 0.41 +/- 0.19 mu m, then the amplitude increased to 2.63 +/- 0.25 mu m. (ii) Left ventricular pressure (LVP) of the heart (n = 7) was reduced by 15 mM BDM from 60.7 /- 2.5 to 2.8 +/- 0.5 mmHg (1 mmHg = 133.3 Pa). LVP increased to 12.8 +/- 1.1 mmHg during subsequent perfusion with 10 mu M RH421 in the presence of BDM and did not change (LVP = 12.4 +/- 2.4 mmHg) during washout of the dye. Therefore, RH421 increased the contractility of rat hearts and isolated myocytes with and without BDM.

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Documento generato il 01/04/20 alle ore 02:09:37