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Titolo:
Transfection of adipocytes by gene gun-mediated transfer
Autore:
Becker, DJ; MacDougald, OA;
Indirizzi:
Univ Michigan, Sch Med, Dept Physiol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 ept Physiol, Ann Arbor, MI 48109 USA
Titolo Testata:
BIOTECHNIQUES
fascicolo: 4, volume: 26, anno: 1999,
pagine: 660 -
SICI:
0736-6205(199904)26:4<660:TOABGG>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN-ALPHA; PARTICLE BOMBARDMENT; CELLS; ADENOVIRUS; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: MacDougald, OA UnivRd,chigan, Sch Med, Dept Physiol, 7620 Med Sci Bldg 2,1301 E Catherine Univ Michigan 7620 Med Sci Bldg 2,1301 E Catherine Rd Ann Arbor MI USA 48109
Citazione:
D.J. Becker e O.A. MacDougald, "Transfection of adipocytes by gene gun-mediated transfer", BIOTECHNIQU, 26(4), 1999, pp. 660

Abstract

Studies of the gene expression, metabolism and intracellular signaling of adipocytes have been hindered by the difficulty of transfecting cultured adipocytes using standard methods. Here, we demonstrate the utility of gene gun-mediated genetic transfer for transfection of differentiated 3T3-Ll adipocytes. Transfection of adipocytes with the Helios(TM) Gene Gun yielded a transfection efficiency that was consistently 5%-10% of total cells. We haveused this technique to overexpress the transcription factor CCAAT/EnhancerBinding Protein alpha (C/EBP alpha) and transactivate an obese gene promoter-luciferase construct. This method has broad utility for transfection of other cell types that are resistant to transfection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:37:52