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Titolo:
Ibd1p, a possible spindle pole body associated protein, regulates nuclear division and bud separation in Saccharomyces cerevisiae
Autore:
Lee, J; Hwang, HS; Kim, J; Song, KW;
Indirizzi:
Yonsei Univ, Coll Sci, Dept Biochem, Seoul 120749, South Korea Yonsei Univ Seoul South Korea 120749 Biochem, Seoul 120749, South Korea ChungnamaNatl Univ, Coll Nat Sci, Dept Microbiol, Taejon 305764, South Kore Chungnam Natl Univ Taejon South Korea 305764 , Taejon 305764, South Kore
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
fascicolo: 3, volume: 1449, anno: 1999,
pagine: 239 - 253
SICI:
0167-4889(19990401)1449:3<239:IAPSPB>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
FISSION YEAST; SCHIZOSACCHAROMYCES-POMBE; SEPTUM FORMATION; BUDDING YEAST; ASSEMBLY CHECKPOINT; ASTRAL MICROTUBULES; MITOTIC SPINDLE; ORIENTATION; CYCLE; GENES;
Keywords:
IBD1; YJR053W; spindle pole body; nuclear division; cytokinesis; bud separation; (Saccharomyces cerevisiae);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Song, KW Yonsei Univ, Coll Sci, Dept Biochem, Seoul 120749, South Korea Yonsei Univ Seoul South Korea 120749 Seoul 120749, South Korea
Citazione:
J. Lee et al., "Ibd1p, a possible spindle pole body associated protein, regulates nuclear division and bud separation in Saccharomyces cerevisiae", BBA-MOL CEL, 1449(3), 1999, pp. 239-253

Abstract

The proper spatial and temporal coordination of mitosis and cytokinesis isessential for maintaining genomic integrity. We describe the identification and characterization of the Saccharomyces cerevisiae IBD1 gene, which encodes a novel protein that regulates the proper nuclear division and bud separation. IBD1 was identified by the limited homology to byr4, a dosage-dependent regulator of cytokinesis in Schizosaccharomyces pombe. IBD1 is not anessential gene, and the knock-out cells show no growth defects except for the reduced mating efficiency [1]. However, upon ectopic expression from aninducible promoter, IBD1 is lethal to the cell and leads to abnormal nuclear division and bud separation. In detail, approximately 90% of the IBD1 overexpressing cells arrest at large bud stages with dividing or divided nuclei. In some IBD1 overexpressing cells, spindle elongation and chromosome separation occur within the mother cell, leading to anucleated and binucleatedaughter cells. The anucleated cell can not bud, but the binucleate cell proceeds through another cell cycle(s) to produce a cell with multiple nuclei and multiple buds. Observations of the F-actin and chitin rings in the IBD1 overexpressing cells reveal that these cells lose the polarity for bud site selection and growth or attain the hyper-polarity for growth. Consistent with the phenotypes, the IBD1 overexpressing cells contain a broad range of DNA content, from 2 to 4 N or more. A functional Ibd1p-GFP fusion protein localizes to a single dot at the nuclear DNA boundary in the divided nuclei or to double dots in dividing nuclei, suggesting its localization on thespindle pole body (SPB). The cross-species expressions of IBD1 in S. pombeand byr4 4 in S. cerevisiae cause defects in shape, implicating the presence of a conserved mechanism for the control of cytokinesis in eukaryotes. We propose that Ibd1p is an SPB associated protein that links proper nucleardivision to cytokinesis and bud separation. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:06:48