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Titolo:
Angiotensin II receptor internalization and signaling in isolated rat hepatocytes
Autore:
Jimenez, E; Caro, MC; Marsigliante, S; Montiel, M;
Indirizzi:
Univ Lecce, Dept Biol, I-73100 Lecce, Italy Univ Lecce Lecce Italy I-73100 iv Lecce, Dept Biol, I-73100 Lecce, Italy Univ Malaga, Fac Med, Dept Bioquim & Biol Mol, Malaga 2980, Spain Univ Malaga Malaga Spain 2980 ept Bioquim & Biol Mol, Malaga 2980, Spain
Titolo Testata:
BIOCHEMICAL PHARMACOLOGY
fascicolo: 10, volume: 57, anno: 1999,
pagine: 1125 - 1131
SICI:
0006-2952(19990515)57:10<1125:AIRIAS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
AGONIST-INDUCED INTERNALIZATION; ADRENAL GLOMERULOSA CELLS; SMOOTH-MUSCLE CELLS; PHOSPHOLIPASE-C; PROTEIN; ENDOCYTOSIS; IDENTIFICATION; GENERATION; ACTIVATION; PATHWAYS;
Keywords:
angiotensin II receptors; angiotensin II receptor internalization; angiotensin II signaling; rat hepatocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Jimenez, E Univ29071,a, Fac Med, Dept Bioquim & Biol Mol, Campus Teatinos S-N, Malaga Univ Malaga Campus Teatinos S-N Malaga Spain 29071 S-N, Malaga
Citazione:
E. Jimenez et al., "Angiotensin II receptor internalization and signaling in isolated rat hepatocytes", BIOCH PHARM, 57(10), 1999, pp. 1125-1131

Abstract

Since angiotensin II (Ang II)-induced receptor internalization is requiredto maintain the production of certain intracellular signals in some targetcells, we investigated thr relationships between Ang II receptor endocytosis and the generation of second messengers in rat hepatocytes. The results of the present study demonstrate that in response to exposure of hepatocytes to Ang II, a decrease in surface Ang II receptors occurred, consistent with a rapid endocytosis of the receptor-bound hormone complex. Pretreatment of cells with okadaic acid (OA) did not have any effect on receptor mediated internalization. In contrast, a marked reduction of the Ang II receptor endocytosis process occurred after treatment of hepatocytes with phenylarsine oxide (PAO), indicating that cysteine residues could be involved in receptor-mediated endocytosis. Stimulation of cells with Ang II blocked the generation of cyclic adenosine monophosphate (cAMP), which follows the stimulation of hepatocytes with forskolin. Moreover, Ang II increased both inositol4,5-bisphosphate (IP2) and inositol 1,4,5-trisphosphate (IP3) generation, and enhanced intercellular calcium concentration ([Ca2+](i)). Exposure of cells to PAO did not alter the effect of Ang II on the accumulation of cAMP after forskolin stimulation, indicating that endocytosis of the agonist-receptor complex is not involved in adenylate cyclase inhibition. Conversely, PAO and OA markedly reduced IP2 and IP3 synthesis, and the plateau phase ofAng II-induced Ca2+ mobilization. The relationship between Anp II-induced endocytosis and the generation of phosphoinositols and increment in [Ca2+](i) indicates that sequestration of the Ang II receptor is necessary re, maintain the production of these intracellular signals in rat hepatocytes. (C)1999 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:10:27