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Titolo:
Isolation and identification of succinic semialdehyde dehydrogenase inhibitory compound from the rhizome of Gastrodia elata Blume
Autore:
Baek, NI; Choi, SY; Park, JK; Cho, SW; Ahn, EM; Jeon, SG; Lee, BR; Bahn, JH; Kim, YK; Shon, IH;
Indirizzi:
Kyung Hee Univ, Dept Life Sci, Yongin Si 449701, Kyunggi Do, South Korea Kyung Hee Univ Yongin Si Kyunggi Do South Korea 449701 gi Do, South Korea Kyunguthe Univ, Inst Life Sci & Resources, Yongin Si 449701, Kyunggi Do, So Kyung Hee Univ Yongin Si Kyunggi Do South Korea 449701 01, Kyunggi Do, So Hallym Univ, Dept Genet Engn, Div Life Sci, Chunchon 200702, South Korea Hallym Univ Chunchon South Korea 200702 ci, Chunchon 200702, South Korea Korea Ginseng & Tobacco Res Inst, Taejon 305345, South Korea Korea Ginseng& Tobacco Res Inst Taejon South Korea 305345 , South Korea Univ Ulsan, Coll Med, Dept Biochem, Seoul 138040, South Korea Univ Ulsan Seoul South Korea 138040 t Biochem, Seoul 138040, South Korea Daelim Ind Co, Daeduk R&D Ctr, Taejon 305345, South Korea Daelim Ind Co Taejon South Korea 305345 Ctr, Taejon 305345, South Korea
Titolo Testata:
ARCHIVES OF PHARMACAL RESEARCH
fascicolo: 2, volume: 22, anno: 1999,
pagine: 219 - 224
SICI:
0253-6269(199904)22:2<219:IAIOSS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRAIN;
Keywords:
Gastrodia elata; anticonvulsant; gastrodin; brain GABA shunt; succinic semialdehyde dehydrogenase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Baek, NI KyungSouthUniv, Dept Life Sci, Seochun Ri 1, Yongin Si 449701, Kyunggi Do, Kyung Hee Univ Seochun Ri 1 Yongin Si Kyunggi Do South Korea 449701
Citazione:
N.I. Baek et al., "Isolation and identification of succinic semialdehyde dehydrogenase inhibitory compound from the rhizome of Gastrodia elata Blume", ARCH PH RES, 22(2), 1999, pp. 219-224

Abstract

In our search for the anticonvulsant constituent of Gastrodia elata repeated column chromatographies guided by activity assay led to isolation of an active compound, which was identified as gastrodin on the basis of spectraldata. Brain succinic semialdehyde dehydrogenase (SSADH) was inactivated bypreincubation with gastrodin in a time-dependent manner and the reaction was monitored by absorption and fluorescence spectroscopic methods. The inactivation followed pseudo-first-order kinetics with the second-rate order constant of 1.2x10(3) M(-1)min(-1). The time course of the reaction was significantly affected by the coenzyme NAD(+), which affected complete protection against the loss of the catalytic activity, whereas substrate succinic semialdehyde failed to prevent the inactivation of the enzyme. It is postulated that the gastrodin is able to elevate the neurotransmitter GABA levels in central nervous system by inhibitory action on one of the GABA degradative enzymes, SSADH.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 23:59:00