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Titolo:
PTRIDENT, A NOVEL VECTOR FAMILY FOR TRICISTRONIC GENE-EXPRESSION IN MAMMALIAN-CELLS
Autore:
FUSSENEGGER M; MAZUR X; BAILEY JE;
Indirizzi:
ETH HONGGERBERG,INST BIOTECHNOL,HPT CH-8093 ZURICH SWITZERLAND ETH HONGGERBERG,INST BIOTECHNOL,HPT CH-8093 ZURICH SWITZERLAND
Titolo Testata:
Biotechnology and bioengineering
fascicolo: 1, volume: 57, anno: 1998,
pagine: 1 - 10
SICI:
0006-3592(1998)57:1<1:PANVFF>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAP-INDEPENDENT TRANSLATION; GREEN FLUORESCENT PROTEIN; VIRUS-RNA TRANSLATION; MESSENGER-RNA; ENCEPHALOMYOCARDITIS VIRUS; INTERNAL INITIATION; ALTERNATIVE TRANSLATION; RETROVIRAL VECTORS; ENTRY SITE; SEQUENCE;
Keywords:
PTRIDENT; TRICISTRONIC EXPRESSION VECTORS; GENE EXPRESSION; MAMMALIAN CELLS; TETRACYCLINE-RESPONSIVE PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
M. Fussenegger et al., "PTRIDENT, A NOVEL VECTOR FAMILY FOR TRICISTRONIC GENE-EXPRESSION IN MAMMALIAN-CELLS", Biotechnology and bioengineering, 57(1), 1998, pp. 1-10

Abstract

We constructed tricistronic expression vectors for the simultaneous and coordinated expression of three independent genes in mammalian cells. One single promoter allows high level and, in some vectors, adjustable transcription of all three cistrons. Whereas the first cistron is translated in a cap-dependent manner, the subsequent ones utilize intercistronic regions of viral origin such as the internal ribosomal entry site of poliovirus or the cap-independent translation enhancer of encephalomyocarditis virus for enhanced translation. Three multiple cloning sites with a total of up to 18 unique restriction sites allow sequential cloning of the genes of interest. The modular structure of thispBluescript(R)-based high copy number vector system allows straightforward movement of individual cistrons among members of the pTRIDENT family, and facilitates their combination with existing expression vectors. (C) 1998 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 14:33:07