Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES IN T-CELL RECEPTOR ALPHA-DEFICIENT MICE WITH CHRONIC COLITIS
Autore:
MIZOGUCHI E; MIZOGUCHI A; CHIBA C; NILES JL; BHAN AK;
Indirizzi:
HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,IMMUNOPATHOL UNIT,DEPT PATHOL,COX 5,100 BLOSSOM ST BOSTON MA 02114 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,IMMUNOPATHOL UNIT,DEPT PATHOL BOSTON MA 02114
Titolo Testata:
Gastroenterology
fascicolo: 6, volume: 113, anno: 1997,
pagine: 1828 - 1835
SICI:
0016-5085(1997)113:6<1828:ACAITR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLAMMATORY BOWEL-DISEASE; PRIMARY SCLEROSING CHOLANGITIS; CHRONIC INTESTINAL INFLAMMATION; ULCERATIVE-COLITIS; MUTANT MICE; CROHNS-DISEASE; B-CELLS; INTRAEPITHELIAL LYMPHOCYTES; NEUTROPHIL AUTOANTIBODIES; BETA-GLUCURONIDASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
E. Mizoguchi et al., "ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES IN T-CELL RECEPTOR ALPHA-DEFICIENT MICE WITH CHRONIC COLITIS", Gastroenterology, 113(6), 1997, pp. 1828-1835

Abstract

Background & Aims: Antineutrophil cytoplasmic antibodies (ANCAs) havebeen detected in approximately 60% of sera from patients with ulcerative colitis. The aim of this study was to examine the presence of ANCAs and distribution of ANCA-producing B cells in the lymphoid tissue ofT-cell receptor alpha-deficient (TCR-alpha(-/-)) mice that develop chronic colitis resembling human ulcerative colitis. Methods: Sera from 87 TCR-alpha(-/-) mice were tested for the presence of ANCAs by enzyme-linked immunosorbent assay against a human neutrophil extract and selected antigens and by immunofluorescence study using human neutrophils. Enzyme-linked immunospot assay was used for detecting ANCA-producingcells from spleen, mesenteric lymph node (MLN), or colon. Results: Approximately 70% of sera from TCR-alpha(-/-) mice showed reactivity against human neutrophil extracts by enzyme-linked immunosorbent assay. Sixty percent of ANCA-positive sera showed a perinuclear reaction pattern. By enzyme-linked immunospot, ANCA-producing cells were detected inMLN and colon and less often in the spleen of TCR-alpha(-/-) mice with chronic colitis. The predominant immunoglobulin isotype of these autoantibodies was immunoglobulin A in the colon but not in the MLN and spleen. Conclusions: TCR-alpha(-/-) mice produce ANCAs. The ANCA (immunoglobulin A isotype)-producing B cells exist primarily in the diseasedcolonic mucosa of TCR alpha(-/-) mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 05:47:23