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Titolo:
TRANSFORMING-GROWTH-FACTOR-BETA RESPONSE AND EXPRESSION IN JUNCTIONALAND ORAL GINGIVAL EPITHELIAL-CELLS
Autore:
LU H; MACKENZIE IC; LEVINE AE;
Indirizzi:
UNIV TEXAS,HLTH SCI CTR,DENT BRANCH,DEPT BASIC SCI,POB 20068 HOUSTON TX 77225 UNIV TEXAS,HLTH SCI CTR,DENT BRANCH,DEPT BASIC SCI HOUSTON TX 77225 DENT SCI INST HOUSTON TX 77225
Titolo Testata:
Journal of Periodontal Research
fascicolo: 8, volume: 32, anno: 1997,
pagine: 682 - 691
SICI:
0022-3484(1997)32:8<682:TRAEIJ>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOKERATIN EXPRESSION; SEQUENCE-ANALYSIS; RETINOIC ACID; II RECEPTOR; CLONING; PROLIFERATION; FIBROBLASTS; INHIBITOR; PATTERNS; PROTEINS;
Keywords:
TGF-BETA-S; TGF-BETA RECEPTORS; RT-PCR; JUNCTIONAL AND ORAL GINGIVAL EPITHELIAL CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
H. Lu et al., "TRANSFORMING-GROWTH-FACTOR-BETA RESPONSE AND EXPRESSION IN JUNCTIONALAND ORAL GINGIVAL EPITHELIAL-CELLS", Journal of Periodontal Research, 32(8), 1997, pp. 682-691

Abstract

The junctional (JE) and oral gingival (OGE) epithelium show distinct morphological phenotypes and express different cell surface and keratin markers. Transforming growth factor-beta (TGF-beta) has been shown to stimulate extracellular matrix formation and inhibit proteolytic matrix degradation in periodontal wound healing. To elucidate potential roles of TGF-beta in gingival epithelial regeneration and reattachment,the present study examined the effects of TGF-beta on JE and OGE cellgrowth and determined the patterns of expression of mRNAs for the TGF-beta isotypes beta 1, beta 2 and beta 3 and TGF-beta receptor types I, II and III. Primary cell cultures were initiated from JE and OGE andthe cell phenotypes confirmed using monoclonal antibodies to specifickeratins. TGF-beta induced a significant growth inhibition in OGE cells derived from 6 different patients with a mean inhibition of 46% anda range of 16-70% (p=0.031). Although responses varied between patients, in general maximum inhibition occurred at 10 ng/ml TGF-beta. JE cells from 5 patients showed no significant growth inhibition by TGF-beta (p=0.125). Greater expression of TGF-beta 2 and receptor type I mRNAwas found in OGE than JE cells and thus appeared to be associated with differentiating epithelial cells. JE cells expressed more TGF-beta type II receptor specific mRNA than did OGE cells, but TGF-beta 1 mRNA expression was similar in JE and OGE cells. JE or OGE cultures derivedfrom 2 of 3 patients showed expression of mRNA for the TGF-beta type III receptor. TGF-beta 3 mRNA was not detected in any of the JE or OGEsamples examined. The greater sensitivity of OGE than JE to the growth inhibiting effects of TGF-beta correlated with higher expression of receptor type I mRNA which, together with the type II receptor, is required for sensitivity to growth inhibition by TGF-beta. The results suggest that, in addition to structural differences, the development of functional differences in the responses of JE and OGE to TGF-beta may be associated with the formation of JE from OGE cells and the reformation of attachment after periodontal surgery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:31:02