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Titolo:
DISSOCIATION BETWEEN THE JORO SPIDER TOXIN SENSITIVITY OF RECOMBINANTLPHA-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONIC ACID RECEPTORS ANDTHEIR ABILITY TO INCREASE INTRACELLULAR CALCIUM
Autore:
MEUCCI O; MILLER RJ;
Indirizzi:
UNIV CHICAGO,DEPT PHARMACOL & PHYSIOL SCI,947 E 58TH ST MC 0926 CHICAGO IL 60637 UNIV CHICAGO,DEPT PHARMACOL & PHYSIOL SCI CHICAGO IL 60637
Titolo Testata:
Neuropharmacology
fascicolo: 10-11, volume: 37, anno: 1998,
pagine: 1431 - 1443
SICI:
0028-3908(1998)37:10-11<1431:DBTJST>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+-PERMEABLE AMPA RECEPTORS; GLUTAMATE-RECEPTOR; CA2+ PERMEABILITY; GLIAL-CELLS; KAINATE RECEPTORS; CHANNELS; SUBUNIT; CYCLOTHIAZIDE; NEURONS; DESENSITIZATION;
Keywords:
JORO SPIDER TOXIN; AMPA RECEPTORS; TOXIN SENSITIVITY; CA PERMEABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
O. Meucci e R.J. Miller, "DISSOCIATION BETWEEN THE JORO SPIDER TOXIN SENSITIVITY OF RECOMBINANTLPHA-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLEPROPIONIC ACID RECEPTORS ANDTHEIR ABILITY TO INCREASE INTRACELLULAR CALCIUM", Neuropharmacology, 37(10-11), 1998, pp. 1431-1443

Abstract

We compared the toxin sensitivity, Ca2+ flux response and rectification properties of recombinant alpha-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptors obtained by transfecting human embryonic kidney (HEK) 293 cells with different ratios of GluR1 and GluR2 cDNAs (10:1 to 1:10). Simultaneous measurements of kainate-activated Ca2fluxes and inward currents, using fura-2 microfluorimetry under voltage clamp conditions, suggested the existence of GluR2 containing channels which are permeable to Ca2+ and insensitive to Joro spider toxin (JSTx). Imaging experiments showed that JSTx inhibition of the Ca2+ response induced by kainate was reduced by increasing the relative amountof GluR2. However, even at GluR1/GluR2(R) ratios of 1:1 and 1:4, cells were still able to flux Ca2+ when stimulated by kainate. GluR2 similarly inhibited the ability of JSTx to reduce kainate-evoked inward currents in whole cell patch-clamp experiments. Variations in the rectification properties of the AMPA currents, induced by changes in the cDNAratio, were not always correlated with the changes in toxin sensitivity and [Ca2+](i) response. Thus, cells with almost linear I-V relationships were partially blocked by JSTx and still Ca2+ permeable. Our results indicate a dissociation between the toxin sensitivity and Ca2+ flux through GluR2 containing AMPA receptors and suggest that receptors with diverse Ca2+ permeabilities are generated by the expression of variable amounts of GluR2. (C) 1998 Elsevier Science Ltd. All rights reserved.

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Documento generato il 13/07/20 alle ore 18:59:38