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Titolo:
FOLLICULAR-FLUID FROM HUMAN OVARIES CONTAINS PLASMA KALLIKREIN IN FREE AND IN COMPLEX WITH A(2)-MACROGLOBULIN
Autore:
OHNISHI J; MURATA M; KOHYAMA K; YOSHIDA H; WADA S; MAKINODA S; FUJIMOTO S; TAKAHASHI T;
Indirizzi:
HOKKAIDO UNIV,GRAD SCH SCI,DIV BIOL SCI SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO UNIV,GRAD SCH SCI,DIV BIOL SCI SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO UNIV,SCH MED,DEPT OBSTET & GYNECOL SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Biomedical research
fascicolo: 2, volume: 18, anno: 1997,
pagine: 161 - 170
SICI:
0388-6107(1997)18:2<161:FFHOCP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; HUMAN GRANULOSA-CELLS; HORMONAL-REGULATION; RAT OVARY; ALPHA-MACROGLOBULINS; TISSUE INHIBITOR; ALPHA-2-MACROGLOBULIN; PREKALLIKREIN; MECHANISM; OVULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
J. Ohnishi et al., "FOLLICULAR-FLUID FROM HUMAN OVARIES CONTAINS PLASMA KALLIKREIN IN FREE AND IN COMPLEX WITH A(2)-MACROGLOBULIN", Biomedical research, 18(2), 1997, pp. 161-170

Abstract

Follicular fluid from human ovaries obtained in in vitro fertilization procedures contained substantial proteinase activities toward synthetic, arginine-containing endopeptidase substrates. Using Pro-Phe-Arg-4-methylcoumaryl-7-amide as a marker for activity, two enzymes, designated human fluid serine proteinase-1 (hFSP-1) and -2 (hFSP-2), were isolated. The molecular weights of intact hFSP-1 and hFSP-2 were estimated to be approximately 90,000 and 730,000, respectively. They exhibitedvirtually identical substrate specificities and inhibition profiles when tested for substrates and inhibitors with low molecular weight. Polypeptide proteinase inhibitors, such as soybean trypsin inhibitor andaprotinin, strongly inhibited hFSP-1, but only inhibited hFSP-2 to a limited extent. These results, together with those of electrophoretic and immunological characterization, revealed that hFSP-1 and hFSP-2 are free plasma kallikrein and the enzyme in complex with the plasma inhibitor alpha(2)-macroglobulin, respectively. hFSP-1 activated human single-chain precursor tissue-type plasminogen activator. This study also indicates that the plasma kallikrein present in follicular fluid probably comes from the liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:45:27