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Titolo:
TELOMERASE COMPONENTS AS A DIAGNOSTIC-TOOL IN HUMAN ORAL LESIONS
Autore:
SUMIDA T; HAMAKAWA H; SOGAWA K; SUGITA A; TANIOKA H; UEDA N;
Indirizzi:
EHIME UNIV,SCH MED,DEPT ORAL SURG SHIGENOBU EHIME 7910295 JAPAN EHIME UNIV,SCH MED,DEPT PATHOL 1 SHIGENOBU EHIME 7910295 JAPAN
Titolo Testata:
International journal of cancer
fascicolo: 1, volume: 80, anno: 1999,
pagine: 1 - 4
SICI:
0020-7136(1999)80:1<1:TCAADI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN TELOMERASE; CANCER; CELLS; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
T. Sumida et al., "TELOMERASE COMPONENTS AS A DIAGNOSTIC-TOOL IN HUMAN ORAL LESIONS", International journal of cancer, 80(1), 1999, pp. 1-4

Abstract

Telomerase activity is considered to be a diagnostic marker of malignancy since most malignant cells express this activity and most somaticcells do not. However, the detection of telomerase activity is rathercomplicated and is affected by many factors. Recently, human telomerase components were cloned and found to consist of 3 subunits. We assessed which component of telomerase best correlates with malignancy in order to study the possibilities for developing a new diagnostic: marker. Telomerase activity was measured by a telomeric repeat amplification protocol (TRAP) assay, and the telomerase components hTR, hTRT-mRNA and TPI-mRNA were detected by the reverse transcriptase-polymerase chain reaction (RT-PCR). Twenty-five of 26 oral malignant lesions, 9 of 22 benign lesions and none of 19 normal control tissues exhibited distinct telomerase activity. hTR and TPI-mRNA expression levels were detected in all malignant lesions and normal control tissues and had no significant correlation with the telomerase activity results. In contrast, hTRT-mRNA expression was closely associated with telomerase activity. All lesions expressing hTRT were telomerase positive. In addition, some samples of dysplastic lesions, benign tumors, lichen planus and normal mucosa exhibiting poor telomerase activity revealed weak expression of hTRT. Expression levels of hTRT-mRNA positively correlated with clinical and pathological findings. Detection of hTRT-mRNA by RT-PCR appeared to be more sensitive for telomerase than measurement of telomerase activity by the TRAP assay. Detection of hTRT-mRNA may provide information useful in the diagnosis of oral malignancies. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 00:36:30