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Titolo:
AN UPDATE ON HIGH-YIELD HEPATOCYTE ISOLATION METHODS AND ON THE POTENTIAL CLINICAL USE OF ISOLATED LIVER-CELLS
Autore:
PUVIANI AC; OTTOLENGHI C; TASSINARI B; PAZZI P; MORSIANI E;
Indirizzi:
UNIV FERRARA,DEPT BIOL,SECT GEN PHYSIOL,VIA L BORSARI 46 I-44100 FERRARA ITALY SANT ANNA UNIV HOSP,DEPT GASTROENTEROL FERRARA ITALY SANT ANNA UNIV HOSP,DEPT SURG FERRARA ITALY
Titolo Testata:
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
fascicolo: 2, volume: 121, anno: 1998,
pagine: 99 - 109
SICI:
1095-6433(1998)121:2<99:AUOHHI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; RAT-LIVER; BIOARTIFICIAL LIVER; PRIMARY CULTURE; HEPATOCELLULAR TRANSPLANTATION; RABBIT HEPATOCYTES; MTT ASSAY; VIABILITY; FAILURE; CYTOTOXICITY;
Keywords:
COLLAGENASE LIVER PERFUSION; ISOLATED HEPATOCYTES; HEPATOCYTE TRANSPLANTATION; HEPATOCYTE-BASED BIOARTIFICIAL LIVER;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
A.C. Puviani et al., "AN UPDATE ON HIGH-YIELD HEPATOCYTE ISOLATION METHODS AND ON THE POTENTIAL CLINICAL USE OF ISOLATED LIVER-CELLS", Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 121(2), 1998, pp. 99-109

Abstract

Isolated hepatocytes are a suitable system for the study of hepatic physiology and metabolism. They are also used for pharmacological and toxicological studies related to hepatic uptake, metabolism, excretion and toxicity of xenobiotics, as well as morphological and metabolic effects induced in the liver as a result of drug or toxic substance exposure. In this paper, the enzymatic methods for hepatocyte isolation insome mammalian species are reviewed, and methods for evaluating cell purification and assessment of cellular morphology and function are also examined. More recently, interest in hepatocyte transplantation hasincreased, and the clinical experimentation of hepatocyte-based liversupport systems has attracted the attention of scientists and hepatologists. From a clinical perspective, using isolated hepatocytes could be useful both for supporting an acutely devastated liver, a chronically diseased liver, and for correcting genetic disorders resulting in metabolically deficient states. Reports of clinical usage of isolated allogenic hepatocytes in hepatocellular transplantation and of xenogenic liver cells in constructing bio-artificial liver support systems arepromising, and are renewing interest in the development of methods for isolation and purification of hepatocytes. (C) 1998 Elsevier ScienceInc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 14:53:22