Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ENHANCED EXPRESSION OF MEMBRANE TYPE-1 MATRIX METALLOPROTEINASE IN MESANGIAL PROLIFERATIVE GLOMERULONEPHRITIS
Autore:
HAYASHI K; OSADA S; SHOFUDA K; HORIKOSHI S; SHIRATO I; TOMINO Y;
Indirizzi:
JUNTENDO UNIV,SCH MED,DEPT MED,DIV NEPHROL,BUNKYO KU,2-1-1 HONGO TOKYO 1130033 JAPAN JUNTENDO UNIV,SCH MED,DEPT MED,DIV NEPHROL,BUNKYO KU TOKYO 1130033 JAPAN INST BIOMED SCI,TERUMO R&D CTR KANAGAWA JAPAN
Titolo Testata:
Journal of the American Society of Nephrology
fascicolo: 12, volume: 9, anno: 1998,
pagine: 2262 - 2271
SICI:
1046-6673(1998)9:12<2262:EEOMTM>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEGRADING NEUTRAL PROTEINASE; PRO-GELATINASE-A; MESSENGER-RNA; EXTRACELLULAR-MATRIX; TRANSMEMBRANE DOMAIN; CELL-PROLIFERATION; BASEMENT-MEMBRANE; GENE-EXPRESSION; IV COLLAGENASE; MT-MMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
K. Hayashi et al., "ENHANCED EXPRESSION OF MEMBRANE TYPE-1 MATRIX METALLOPROTEINASE IN MESANGIAL PROLIFERATIVE GLOMERULONEPHRITIS", Journal of the American Society of Nephrology, 9(12), 1998, pp. 2262-2271

Abstract

Matrix metalloproteinase-2 (MMP-2, gelatinase A) is involved in the inflammatory and sclerotic events of glomerular diseases. Newly identified membrane-type matrix metalloproteinases (MT-MMP) have been shown to activate specifically proMMP-2. To date, several types of MT-MMP have been cloned; however, their expressions in glomerular diseases have not been evaluated. To investigate the role of MT-MMP in glomerular diseases, the glomerular gene expression and enzymatic activity of MT-MMP were examined during the time course of nephritis induced in rats byanti-Thy1.1 antibody injection. Both MT1-MMP and MMP-2 mRNA expression increased prominently 5 and 10 d after anti-Thy1.1 antibody injection and decreased thereafter, as assayed by semiquantitative reverse transcription-PCR. In contrast, there were no remarkable changes in the gene expression of MT2-MMP between normal and diseased tissue, and thatof MT3-MMP was not detected in isolated glomeruli by reverse transcription-PCR analysis. The activation of proMMP-2 as analyzed by gelatin zymography correlated with the glomerular MT1-MMP gene expression, suggesting that proMMP-2 was activated by MT1-MMP. Protein and mRNA expression of fibronectin, one of the major mesangial matrix proteins and substrate of MMP-2, were also synchronized with MT1-MMP and MMP-2 expression. In situ hybridization revealed intense MT1-MMP mRNA expression in the proliferating mesangial cells. Interestingly, MT1-MMP gene expression exhibited a similar distribution as alpha-smooth muscle actin expression, which was closely associated with mesangial phenotypic change. These results suggest that among the newly identified MT-MMP, MT1-MMP may play the central role in activation of proMMP-2. Furthermore, the enhancement of MT1-MMP and MMP-2 expression associated with mesangial phenotypic change may contribute to the development of anti-Thy1.1antibody-induced glomerulonephritis and remodeling of extracellular matrices.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:57:09