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Titolo:
PROTEOGLYCAN DISTRIBUTION DURING HEALING OF CORNEAL STROMAL WOUNDS INCHICK
Autore:
SUNDARRAJ N; FITE D; BELAK R; SUNDARRAJ S; RADA J; OKAMOTO S; HASSELL J;
Indirizzi:
SHRINERS HOSP CRIPPLED CHILDREN,12502 N PINE DR TAMPA FL 33612 SHRINERS HOSP CRIPPLED CHILDREN TAMPA FL 33612 UNIV S FLORIDA TAMPA FL 00000 UNIV PITTSBURGH,SCH MED PITTSBURGH PA 00000 UNIV N DAKOTA GRAND FORKS ND 58201 YOSANOUMI HOSP KYOTO JAPAN
Titolo Testata:
Experimental Eye Research
fascicolo: 4, volume: 67, anno: 1998,
pagine: 433 - 442
SICI:
0014-4835(1998)67:4<433:PDDHOC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATAN SULFATE PROTEOGLYCAN; COLLAGEN TYPE-I; HEPARAN-SULFATE; IMMUNOHISTOCHEMICAL EVALUATION; EXTRACELLULAR MATRICES; SIGNAL-TRANSDUCTION; BASEMENT-MEMBRANE; VI COLLAGEN; RABBIT; GLYCOSAMINOGLYCANS;
Keywords:
CHICK CORNEA; WOUND HEALING; CORNEA; PROTEOGLYCANS; CORNEAL STROMA; KERATOCAN; LUMICAN; PERLECAN; KERATAN SULFATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
N. Sundarraj et al., "PROTEOGLYCAN DISTRIBUTION DURING HEALING OF CORNEAL STROMAL WOUNDS INCHICK", Experimental Eye Research, 67(4), 1998, pp. 433-442

Abstract

Proteoglycan distribution during corneal stromal healing in growing corneas of young chicks were histologically and immunohistochemically analysed. Single linear incisions to produce partial-thickness wounds were made in the corneas of 5 day old chicks. The corneas were harvested at different times after wounding and processed for either histochemical analyses using periodic acid-Schiffs reaction (PAS) or for indirect immunofluorescence analyses of lumican, keratocan, keratan sulfate,perlecan and laminin. Linear corneal stromal incisions were completely covered by migrated stratified epithelium by day 2 post wounding andresulted in a gaping wound with a thinner stroma. New stromal scar tissue formed between the epithelium and the original stroma that resulted in partial restoration of stromal thickness. During the first two to three weeks of healing, the stromal tissue filling the depression formed from the gaping wound, was hypercellular and PAS positive, indicating significantly higher levels of glycoprotein content but no new Bowman's membrane was formed. By four weeks, the scar tissue occupied a 2-3 mm wide region. Immunofluorescence analyses indicated that other major differences in the healing and normally growing stroma were the increased synthesis and deposition of perlecan and laminin. No differences were evident in the immunfluorescence for keratocan or keratan sulfate in the scar tissue, but the scar tissue did contain markedly decreased levels of lumican. Thus, the regulation of proteoglycan and glycoprotein synthesis is altered in the keratocytes that are recruited tothe wounded regions in the growing corneal stroma of post-hatched young chicks. While synthesis and deposition of adhesive molecules including laminin and perlecan are elevated, the synthesis of one of the keratan sulfate proteoglycans, lumican, is reduced in the scar tissue as compared to the normally growing stroma. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 19:58:44