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Titolo:
VERTICAL TRANSMISSION OF CITROBACTER-DIVERSUS DOCUMENTED BY DNA-FINGERPRINTING
Autore:
HARVEY BS; KOEUTH T; VERSALOVIC J; WOODS CR; LUPSKI JR;
Indirizzi:
BAYLOR COLL MED,DEPT MOLEC & HUMAN GENET,1 BAYLOR PLAZA HOUSTON TX 77030 BAYLOR COLL MED,DEPT MOLEC & HUMAN GENET HOUSTON TX 77030 BAYLOR COLL MED,DEPT PEDIAT HOUSTON TX 77030 UNIV TEXAS,HLTH SCI CTR,DEPT PEDIAT HOUSTON TX 77225 WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT PEDIAT WINSTON SALEM NC 27103
Titolo Testata:
Infection control and hospital epidemiology
fascicolo: 10, volume: 16, anno: 1995,
pagine: 564 - 569
SICI:
0899-823X(1995)16:10<564:VTOCDB>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; BRAIN-ABSCESS; MOLECULAR EPIDEMIOLOGY; NOSOCOMIAL MENINGITIS; NEONATAL MENINGITIS; SEQUENCES; STRAINS; GENOMES; VENTRICULITIS; OUTBREAK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
B.S. Harvey et al., "VERTICAL TRANSMISSION OF CITROBACTER-DIVERSUS DOCUMENTED BY DNA-FINGERPRINTING", Infection control and hospital epidemiology, 16(10), 1995, pp. 564-569

Abstract

OBJECTIVE: To confirm the vertical transmission of Citrobacter diversus from a mother to her infant and to evaluate the epidemiologic usefulness of a new automated procedure for analysis of polymerase chain reaction (PCR)-generated DNA fingerprints. DESIGN: Repetitive element-based PCR (rep-PCR) analysis of C diversus isolates from the blood and amniotic fluid of a mother and the blood of her infant was performed. Unrelated C diversus isolates also were characterized and compared withthe isolates from mother and infant. DNA fingerprints were generated by gel electrophoresis of PCR products derived from either unlabeled standard repetitive sequence-based oligonucleotide primers or fluorescent primers. The standard rep-PCR fingerprints were analyzed by visual inspection. The fluorescent primers were used in fluorophore-enhanced rep-PCR (FERP), and the FERP DNA fingerprints were analyzed by an Applied BioSystems (ABT) Model 373A laser scanning unit equipped with Genescan 672 software (Applied Biosystems, Inc, Foster City, CA). SETTING AND PATIENTS: a mother and her newborn infant, both with invasive disease due to C diversus, in an urban tertiary-care hospital. RESULTS: The DNA fingerprints of the maternal blood, amniotic fluid, and infant blood isolates of C diversus were identical by both visual inspection of ethidium bromide-stained agarose gels and computer-aided analysis ofFERP patterns. These strains appeared to differ from all but one control isolate, which had been collected 7 years earlier in the same cityin which the infant was born. CONCLUSIONS: Vertical transmission of Cdiversus from mother to infant can occur in utero. Automated analysisof rep-PCR-generated DNA fingerprints derived using fluorescent primers is an objective means for comparing isolates of C diversus and in all likelihood would be useful for other species of bacteria that possess repetitive elements (Infect Control Hosp Epidemiol 1995;16:564-569).

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Documento generato il 02/12/20 alle ore 05:15:26