Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SPECIFIC ANTIGENS OF CHLAMYDIA-PECORUM AND THEIR HOMOLOGS IN CHLAMYDIA-PSITTACI AND CHLAMYDIA-TRACHOMATIS
Autore:
BAGHIAN A; KOUSOULAS K; TRUAX R; STORZ J;
Indirizzi:
LOUISIANA STATE UNIV,SCH VET MED,DEPT VET MICROBIOL & PARASITOL BATONROUGE LA 70803
Titolo Testata:
American journal of veterinary research
fascicolo: 12, volume: 57, anno: 1996,
pagine: 1720 - 1725
SICI:
0002-9645(1996)57:12<1720:SAOCAT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER-MEMBRANE PROTEIN; MONOCLONAL-ANTIBODIES; SP-NOV; EPITOPES; IDENTIFICATION; PNEUMONIAE; DIVERSITY; ORIGIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
A. Baghian et al., "SPECIFIC ANTIGENS OF CHLAMYDIA-PECORUM AND THEIR HOMOLOGS IN CHLAMYDIA-PSITTACI AND CHLAMYDIA-TRACHOMATIS", American journal of veterinary research, 57(12), 1996, pp. 1720-1725

Abstract

Objective-To analyze the antigenic cross reactivity of various proteins of strains of Chlamydia pecorum, C psittaci, and C trachomatis. Samples and Procedures-Strains FC-Stra and LW-613 of C pecorum; strains B577, Fitz-9, and 6BC of C psittaci, and strain LGV-2 of C trachomatis were studied. Strains of C pecorum were propagated in Georgia bovine kidney cells, and other chlamydial strains were propagated in L cells or Georgia bovine kidney cells. Partially purified chlamydial elementary bodies propagated in RAG cells, a BALB/c cell line cloned from a renal adenocarcinoma of BALB/c mice, were used to immunize BALB/c mice for production of monoclonal antibodies. Rabbits were inoculated with yolk sack-propagated, purified elementary bodies to produce polyclonal antisera. The reaction of monoclonal antibodies and polyclonal antiserawith chlamydial proteins was analyzed by use of immunoblot techniques. Results-Two monoclonal antibodies reacted with a 90-kd protein of C psittaci and a 94-kd protein of C pecorum strains. One monoclonal antibody reacted strongly with a 67-kd protein of C pecorum and strain B577 of C psittaci, but weakly with proteins of strains 6BC and LGV-2. Another monoclonal antibody reacted with a 46-kd protein of 2 C pecorum strains and of strain B577 of C psittaci, but not with those of strains 6BC and LGV-2. Two monoclonal antibodies reacted with a 20-kd protein of C pecorum and a 22-kd protein of C psittaci and LGV-2 strains. Polyclonal antisera reacted similarly with the proteins identified by monoclonal antibodies in the various chlamydial strains. Conclusions-Reactions of several monoclonal antibodies with chlamydial antigens indicated that 67- and 46-kd proteins contain genus- and species-specific epitopes, respectively; a 94-kd protein of C pecorum is homologous to a90-kd protein of C psittaci and C trachomatis strains; and a 20-kd protein of C pecorum corresponds to a 22-kd protein of C psittaci and C trachomatis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:47:58