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Titolo:
PROMOTING DETACHMENT OF NEUTROPHILS ADHERENT TO MURINE POSTCAPILLARY VENULES TO CONTROL INFLAMMATION - EFFECT OF LIPOCORTIN 1
Autore:
LIM LHK; SOLITO E; RUSSOMARIE F; FLOWER RJ; PERRETTI M;
Indirizzi:
UNIV LONDON ST BARTHOLOMEWS HOSP MED COLL,COLL MED,WILLIAM HARVEY RESINST LONDON EC1M 6BQ ENGLAND UNIV LONDON ST BARTHOLOMEWS HOSP MED COLL,COLL MED,WILLIAM HARVEY RESINST LONDON EC1M 6BQ ENGLAND INST COCHIN GENET MOL,U332 INSERM F-75014 PARIS FRANCE
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 24, volume: 95, anno: 1998,
pagine: 14535 - 14539
SICI:
0027-8424(1998)95:24<14535:PDONAT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD MONONUCLEAR LEUKOCYTES; N-TERMINUS; ANNEXIN-V; ADHESION; INVIVO; INHIBITION; MODULATION; PROTEINS; DEXAMETHASONE; RECRUITMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
L.H.K. Lim et al., "PROMOTING DETACHMENT OF NEUTROPHILS ADHERENT TO MURINE POSTCAPILLARY VENULES TO CONTROL INFLAMMATION - EFFECT OF LIPOCORTIN 1", Proceedings of the National Academy of Sciences of the United Statesof America, 95(24), 1998, pp. 14535-14539

Abstract

In this study we investigated, using intravital microscopy, how neutrophil extravasation across mouse mesenteric postcapillary venules is inhibited by the glucocorticoid-regulated protein lipocortin (LC; also termed annexin) 1, Intraperitoneal injection of 1 mg of zymosan into mice induced neutrophil rolling on the activated mesenteric endotheliumfollowed by adhesion (maximal at 2 hr: 5-6 cells per 100-mu m of vessel length) and emigration (maximal at 4 hr: 8-10 cells per high powered field). Treatment of mice with human recombinant LC1 (2 mg/kg s.c.) or its mimetic peptide Ac2-26 (13 mg/kg s.c.) did not modify cell rolling but markedly reduced (greater than or equal to 50%) the degree of neutrophil adhesion and emigration (P < 0.05). Intravenous treatment with peptide Ac2-26 (13 mg/kg) or recombinant human LC1 (0.7-2 mg/kg) promoted detachment of neutrophils adherent to the endothelium 2 hr after zymosan administration, with adherent cells detaching within 4.12 +/- 0.75 min and 2.36 +/- 0.31 min, respectively (n = 20-25 cells). Recruitment of newly adherent cells to the endothelium was unaffected. The structurally related protein LC5 was inactive in this assay, whereasa chimeric molecule constructed from the N terminus of LC1 (49 aa) attached to the core region of LC5 produced cell detachment with kinetics similar to LC1, Removal of adherent neutrophils from activated postcapillary endothelium is a novel pharmacological action, and it is at this site where LC1 and its mimetics operate to down-regulate this aspect of the host inflammatory response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 13:45:06