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Titolo:
CHOLECYSTOKININ-INDUCED DESENSITIZATION, RECEPTOR PHOSPHORYLATION, AND INTERNALIZATION IN THE CHP212 NEUROBLASTOMA CELL-LINE
Autore:
RAO RV; HADAC EM; ROETTGER BF; MILLER LJ;
Indirizzi:
MAYO CLIN & MAYO FDN,CTR BASIC RES DIGEST DIS,200 1ST ST SW ROCHESTERMN 55905 MAYO CLIN & MAYO FDN,CTR BASIC RES DIGEST DIS ROCHESTER MN 55905 MAYO CLIN & MAYO FDN,DEPT BIOCHEM & MOL BIOL ROCHESTER MN 55905
Titolo Testata:
Journal of neurochemistry
fascicolo: 6, volume: 68, anno: 1997,
pagine: 2356 - 2362
SICI:
0022-3042(1997)68:6<2356:CDRPA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC ACINAR CELL; G-PROTEIN; SECRETION;
Keywords:
RECEPTOR INTERNALIZATION; RECEPTOR PHOSPHORYLATION; G PROTEIN-COUPLED RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
R.V. Rao et al., "CHOLECYSTOKININ-INDUCED DESENSITIZATION, RECEPTOR PHOSPHORYLATION, AND INTERNALIZATION IN THE CHP212 NEUROBLASTOMA CELL-LINE", Journal of neurochemistry, 68(6), 1997, pp. 2356-2362

Abstract

Agonist stimulation of cells often results in desensitization of the response, to protect the cell from overstimulation. We have previouslyshown that the type A cholecystokinin (CCK) receptor on the pancreatic acinar cell and on the model CHO-CCKR cell line undergoes desensitization in response to CCK, with receptor phosphorylation and internalization playing key roles. Although these mechanisms contribute in a cell-specific manner, no analogous information exists for the CCK receptor expressed on neuronal cells, where in vivo data demonstrate a particularly sensitive response to CCK. The present study was designed to explore CCK receptor desensitization in the CHP212 neuroblastoma cell line, focusing on inositol 1,4,5-trisphosphate (IP3) responses to CCK and on recognized molecular and cellular mechanisms of desensitization. CCK promptly stimulated IF, responses in these cells, with hormonal responsiveness rapidly and completely desensitized. Both receptor phosphorylation and internalization were observed to occur, with the former occurring most rapidly and likely being responsible for the earliest desensitization observed. Although the time course of receptor phosphorylation and dephosphorylation, and the groups of kinases involved in the neuroblastoma cell line, were most similar to those in the pancreatic cell, the movement of the agonist-bound receptor in these cells wasquite different from that in the pancreatic cell and most similar to that in the CHO-CCKR cell line. This hybrid response supports the cell-specific nature of CCK receptor regulation and provides an important system to explore the molecular determinants of these processes.

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Documento generato il 02/04/20 alle ore 02:48:39