Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
REGULATION OF CORTICOTROPIN-RELEASING FACTOR-RECEPTOR FUNCTION IN HUMAN Y-79 RETINOBLASTOMA CELLS - RAPID AND REVERSIBLE HOMOLOGOUS DESENSITIZATION BUT PROLONGED RECOVERY
Autore:
HAUGER RL; DAUTZENBERG FM; FLACCUS A; LIEPOLD T; SPIESS J;
Indirizzi:
UNIV CALIF SAN DIEGO,DEPT PSYCHIAT,9500 GILMAN DR LA JOLLA CA 92093 VET ADM MED CTR LA JOLLA CA 90034 MAX PLANCK INST EXPT MED,DEPT MOL NEUROENDOCRINOL D-3400 GOTTINGEN GERMANY
Titolo Testata:
Journal of neurochemistry
fascicolo: 6, volume: 68, anno: 1997,
pagine: 2308 - 2316
SICI:
0022-3042(1997)68:6<2308:ROCFFI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; FACTOR CRF RECEPTORS; IMMOBILIZATION STRESS; ARGININE-VASOPRESSIN; ADENYLYL-CYCLASE; DOWN-REGULATION; RAT-BRAIN; PITUITARY; HORMONE; RESPONSES;
Keywords:
CORTICOTROPIN-RELEASING FACTOR; RECEPTOR DESENSITIZATION; CYCLIC AMP; CORTICOTROPIN-RELEASING FACTOR RECEPTOR MESSENGER-RNA; STRESS; RETINOBLASTOMA CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
R.L. Hauger et al., "REGULATION OF CORTICOTROPIN-RELEASING FACTOR-RECEPTOR FUNCTION IN HUMAN Y-79 RETINOBLASTOMA CELLS - RAPID AND REVERSIBLE HOMOLOGOUS DESENSITIZATION BUT PROLONGED RECOVERY", Journal of neurochemistry, 68(6), 1997, pp. 2308-2316

Abstract

Homologous receptor desensitization is an important regulatory response to continuous activation by agonist that involves the uncoupling ofa receptor from its G protein. When human retinoblastoma Y-79 cells expressing corticotropin-releasing factor (CRF) receptors were preincubated with CRF for 10 min-4 h, a time-dependent reduction in both the peak and sensitivity of CRF-stimulated intracellular cyclic AMP (cAMP) accumulation developed with a t(1/2) of 38 min and an EC50 of 6-7 nM CRF. CRF receptor desensitization was slowly reversible after a 4-h CRFpreincubation with a t(1/2) of 13 h and a full restoration of cAMP responsiveness to CRF at 24 h following the removal of 10 nM CRF. Because the ability of vasoactive intestinal peptide, forskolin, or (-)-isoproterenol to stimulate cAMP accumulation was not diminished in Y-79 cells desensitized with 10 nM CRF, the observed desensitization was considered to be a specific homologous action of CRF. CRF receptor desensitization was markedly attenuated by CRF receptor antagonists, which alone did not produce any appreciable reduction in CRF-stimulated cAMP accumulation. Although recent reports have demonstrated a rapid declinein steady-state levels of CRF receptor type 1 (CRF-R1) mRNA in anterior pituitary cells during several hours of exposure to CRF, there was no observed reduction in CRF-R1 mRNA levels when Y-79 cells were preincubated with 10 nM CRF for 10 min-24 h despite a rapid time- and concentration-dependent loss of CRF receptors from the retinoblastoma cell surface.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:46:17