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Titolo:
STRUCTURAL REQUIREMENTS FOR THE CYTOTOXICITY OF THE N-TERMINAL REGIONOF HIV TYPE-1 NEF
Autore:
CURTAIN CC; LOWE MG; MACREADIE IG; GENTLE IR; LAWRIE GA; AZAD AA;
Indirizzi:
BIOMOL RES INST,343 ROYAL PARADE PARKVILLE VIC 3052 AUSTRALIA UNIV QUEENSLAND,DEPT CHEM ST LUCIA QLD 4072 AUSTRALIA
Titolo Testata:
AIDS research and human retroviruses
fascicolo: 17, volume: 14, anno: 1998,
pagine: 1543 - 1551
SICI:
0889-2229(1998)14:17<1543:SRFTCO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; CELLULAR SERINE KINASE; DOWN-REGULATION; MUTATIONAL ANALYSIS; T-LYMPHOCYTES; SURFACE CD4; AMINO-ACID; PROTEIN; MELITTIN; ASSOCIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
C.C. Curtain et al., "STRUCTURAL REQUIREMENTS FOR THE CYTOTOXICITY OF THE N-TERMINAL REGIONOF HIV TYPE-1 NEF", AIDS research and human retroviruses, 14(17), 1998, pp. 1543-1551

Abstract

We have found that the hemolytic and cytotoxic activities of myristoylated Nef N-terminal peptides require a net positive charge in the first seven amino residues of the sequence. The activities are considerably less dependent on the secondary structure of the peptides, Film balance studies showed that both active and inactive peptides interacted with neutral phospholipid monolayers, suggesting that binding to neutral lipids was not a sufficient condition for lytic activity. It was also found that nonmyristoylated N-terminal peptide did not interact to the same extent with the monolayer, indicating that myristoylation wasessential for lipid interaction. It is considered that the positivelycharged residues of the proximate N terminus of Nef interact with acidic lipids of biological membranes, reinforcing the weak membrane-targeting properties of the myristyl chain. Parallels are drawn between this mode of interaction with membranes and that of members of the Src family of proteins, which are also myristoylated and have positively charged residues in their proximate N termini, In particular, these proteins and Nef also have serine residues in their proximal N-terminal regions, which when phosphorylated could neutralize the positive charge and thus provide a mechanism for modulating membrane interaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 05:57:19