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Titolo:
DISTINCT PATTERNS OF EXPRESSION AND PHYSIOLOGICAL-EFFECTS OF SST1 ANDSST2 RECEPTOR SUBTYPES IN MOUSE HYPOTHALAMIC NEURONS AND ASTROCYTES IN CULTURE
Autore:
VIOLLET C; LANNEAU C; FAIVREBAUMAN A; ZHANG J; DJORDJIJEVIC D; LOUDES C; GARDETTE R; KORDON C; EPELBAUM J;
Indirizzi:
CTR PAUL BROCA,U159 INSERM,2TER RUE ALESIA F-75014 PARIS FRANCE CTR PAUL BROCA,U159 INSERM F-75014 PARIS FRANCE
Titolo Testata:
Journal of neurochemistry
fascicolo: 6, volume: 68, anno: 1997,
pagine: 2273 - 2280
SICI:
0022-3042(1997)68:6<2273:DPOEAP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLONED SOMATOSTATIN RECEPTORS; BINDING-SITES; DIFFERENTIAL EXPRESSION; TRANSIENT EXPRESSION; ADENYLYL-CYCLASE; SPINAL-CORD; RAT-BRAIN; AFFINITY; CEREBELLUM; IDENTITY;
Keywords:
QUANTITATIVE REVERSE TRANSCRIPTION; POLYMERASE CHAIN REACTION; RADIORECEPTOR BINDING; ADENYLYL CYCLASE; PATCH CLAMP; OCTREOTIDE; CH-275;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
C. Viollet et al., "DISTINCT PATTERNS OF EXPRESSION AND PHYSIOLOGICAL-EFFECTS OF SST1 ANDSST2 RECEPTOR SUBTYPES IN MOUSE HYPOTHALAMIC NEURONS AND ASTROCYTES IN CULTURE", Journal of neurochemistry, 68(6), 1997, pp. 2273-2280

Abstract

Somatostatin (SRIF) receptor subtypes (sst) were characterized in hypothalamic neurons and astrocytes by quantitative reverse transcription-polymerase chain reaction and radioreceptor assays using [I-125-Tyr(0),D-Trp(8)]SRIF-14 as a ligand in ionic conditions discriminating between SRIF-1 (sst2, -3, and -5 receptors) and SRIF-2 (sst1 and -4 receptors) binding sites. In neurons, sst1 mRNA levels were twofold higher than those of sst2, and sst3-5 expression was only minor. Astrocytes expressed 10-fold less sst mRNAs than neurons, which corresponded mostly(80%) to sst2. SRIF-1 binding site radioautography indicated that 10%of hypothalamic neurons were labelled on both cell bodies and neuritic processes, as were 35% of astrocytes. On neuronal and glial membranes, SRIF-14 and octreotide, an sst2/sst3/ sst5-selective analogue, completely displaced SRIF-1 binding, whereas des-AA(1,2,5)[D-Trp(8),IAmp(9)]SRIF (CH-275), an sst1-selective analogue, was ineffective. Using SRIF-2 conditions, only SRIF-14 and CH-275 displaced the binding on neurons. No SRIF-2 binding was observed on glia. SRIF-14 and octreotide inhibited forskolin-stimulated adenylyl cyclase activity in neurons and glia, whereas CH-275 was effective in neurons only. In patch-clamp experiments, SRIF-14 modulated the glutamate sensitivity of hypothalamic neurons with either synergistic or antagonistic effects; CH-275 was only stimulatory and octreotide inhibitory. It is concluded that hypothalamic neurons express primarily sst1 and sst2, sst2 predominates in astrocytes, and both receptors induce distinct biological effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:36:43