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Titolo:
PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE EXPRESSION AND MODULATION OF NEURONAL EXCITABILITY IN GUINEA-PIG CARDIAC GANGLIA
Autore:
BRAAS KM; MAY V; HARAKALL SA; HARDWICK JC; PARSONS RL;
Indirizzi:
UNIV VERMONT,COLL MED,DEPT ANAT & NEUROBIOL,GIVEN HLTH SCI COMPLEX BURLINGTON VT 05405 UNIV VERMONT,COLL MED,DEPT ANAT & NEUROBIOL BURLINGTON VT 05405
Titolo Testata:
The Journal of neuroscience
fascicolo: 23, volume: 18, anno: 1998,
pagine: 9766 - 9779
SICI:
0270-6474(1998)18:23<9766:PACPEA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE INTESTINAL POLYPEPTIDE; DIFFERENTIAL SIGNAL-TRANSDUCTION; TERMINAL EXTRACELLULAR DOMAIN; MOLECULAR-CLONING; PACAP RECEPTOR; FUNCTIONAL EXPRESSION; NEUROPEPTIDE-Y; PROOPIOMELANOCORTIN EXPRESSION; CHOLINE-ACETYLTRANSFERASE; INTRACARDIAC NEURONS;
Keywords:
PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE; VASOACTIVE INTESTINAL PEPTIDE; PACAP RECEPTOR; CARDIAC GANGLIA; PARASYMPATHETIC GANGLIA; NEUROPEPTIDE RECEPTORS; AUTONOMIC NERVOUS SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
K.M. Braas et al., "PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE EXPRESSION AND MODULATION OF NEURONAL EXCITABILITY IN GUINEA-PIG CARDIAC GANGLIA", The Journal of neuroscience, 18(23), 1998, pp. 9766-9779

Abstract

Cardiac output is regulated by the coordinate interactions of stimulatory sympathetic and inhibitory parasympathetic signals. Intracardiac parasympathetic ganglia are integrative centers of cardiac regulation,and modulation of the parasympathetic drive on the heart is accomplished by altering intrinsic cardiac ganglion neuron excitability. The pituitary adenylate cyclase-activating polypeptide (PACAP)/vasoactive intestinal peptide (VIP) family of peptides modulates cardiac function, and in guinea pig heart, PACAP appears to act directly on intrinsic parasympathetic cardiac ganglia neurons through PACAP-selective receptors. A multidisciplinary project tested whether cardiac PACAP peptides act through PACAP-selective receptors as excitatory neuromodulators amplifying the parasympathetic inhibition from guinea pig cardiac ganglia. The in vivo sources of regulatory PACAP peptides were localized immunocytochemically to neuronal fibers and a subpopulation of intrinsic postganglionic cardiac neurons. RT-PCR confirmed that cardiac ganglia expressed proPACAP transcripts and have PACAP peptide biosynthetic capabilities. Messenger RNA encoding PACAP-selective PAC1 receptor isoforms were also present in cardiac ganglia. Alternative splicing of PAC1 receptor transcripts produced predominant expression of the very short variant with neither HIP nor HOP cassettes; lower levels of the PAC1HOP2 receptor mRNA were present. Almost all of the parasympathetic neurons expressed membrane-associated PAC1 receptor proteins, localized immunocytochemically, which correlated with the population of cells that responded physiologically to PACAP peptides. PACAP depolarized cardiacganglia neurons and increased neuronal membrane excitability. The rank order of peptide potency on membrane excitability in response to depolarizing currents was PACAP27>PACAP38>VIP. The PACAP-induced increasein excitability was not a function of membrane depolarization nor wasit caused by alterations in action potential configuration. These results support roles for PACAP peptides as integrative modulators amplifying, through PACAP-selective receptors, the parasympathetic cardiac ganglia inhibition of cardiac output.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:32:39