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Titolo:
ANALYSIS OF LACROSSE-VIRUS-S MESSENGER-RNA 5' TERMINI IN INFECTED MOSQUITO CELLS AND AEDES TRISERIATUS MOSQUITOS
Autore:
DOBIE DK; BLAIR CD; CHANDLER LJ; RAYMSKELLER A; MCGAW MM; WASIELOSKI LP; BEATY BJ;
Indirizzi:
COLORADO STATE UNIV,DEPT MICROBIOL,ARTHROPOD BORNE & INFECT DIS LAB FT COLLINS CO 80523 COLORADO STATE UNIV,DEPT MICROBIOL,ARTHROPOD BORNE & INFECT DIS LAB FT COLLINS CO 80523
Titolo Testata:
Journal of virology
fascicolo: 6, volume: 71, anno: 1997,
pagine: 4395 - 4399
SICI:
0022-538X(1997)71:6<4395:AOLM5T>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA TRANSCRIPTION PROCESSES; HARE BUNYAVIRUS S; MESSENGER-RNA; SEGMENT; PROTEIN; REPLICATION; POLYMERASE; SEQUENCES; VIRIONS; ENDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
D.K. Dobie et al., "ANALYSIS OF LACROSSE-VIRUS-S MESSENGER-RNA 5' TERMINI IN INFECTED MOSQUITO CELLS AND AEDES TRISERIATUS MOSQUITOS", Journal of virology, 71(6), 1997, pp. 4395-4399

Abstract

Nucleotide sequences were determined for the 5' termini of La Crosse virus (LAC) S segment mRNA from persistently infected mosquito cell cultures (C6/36 from Aedes albopictus) and embryos (Aedes triseriatus). LAC primes transcription of its mRNA with ''scavenged'' 5' caps and adjacent oligonucleotides from host mRNAs, and these non-virus-encoded 5'-terminal extensions are heterogeneous in infected mammalian cells. The nature of mosquito host-derived primers has not been previously investigated. During early C6/36 cell infection, LAC mRNA 5'-terminal sequences were heterogeneous, but variability decreased as infection persisted. One predominant sequence, 5' CCACTCGCCACT (sequence 1), was observed throughout C6/36 tell infection but was more prevalent after 15 days postinfection. This LAC mRNA 5'-terminal sequence comprised 81% of the scavenged host oligonucleotides from vertically infected A. triseriatus eggs during embryogenesis. As these embryos progressed in the dormant overwintering stage (diapause), the predominant scavenged sequence became 5' AGGAAAAGATGGT (sequence 2), and sequence 1 became less prevalent. As the eggs emerged from diapause, the LAC mRNA 5' termini were more variable; 33% had sequence 1, and the remainder were heterogeneous. In post-diapausing eggs, 100% of viral mRNAs had sequence 1 attheir 5' termini. Molecular analyses thus revealed continuous but selective LAC cap scavenging during persistent C6/36 cell infection and during embryogenesis and diapause in A. triseriatus eggs. The variety of host-derived sequences was limited in both biosynthetically active (embryonating) and dormant (diapausing) eggs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:33:03