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Titolo:
CHANGES IN ORGANIZATION OF CRITHIDIA-FASCICULATA KINETOPLAST DNA-REPLICATION PROTEINS DURING THE CELL-CYCLE
Autore:
JOHNSON CE; ENGLUND PT;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,725 N WOLFE ST BALTIMORE MD21205 JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205
Titolo Testata:
The Journal of cell biology
fascicolo: 4, volume: 143, anno: 1998,
pagine: 911 - 919
SICI:
0021-9525(1998)143:4<911:CIOOCK>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASE-EXCISION-REPAIR; MITOCHONDRIAL-DNA; POLYMERASE-BETA; BOVINE TESTIS; 2 SITES; MINICIRCLES; NETWORKS; NUCLEAR; LOCALIZATION; TOPOISOMERASE;
Keywords:
CELL CYCLE; KINETOPLAST DNA; DNA REPLICATION; DNA POLYMERASE BETA; TOPOISOMERASE II;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
C.E. Johnson e P.T. Englund, "CHANGES IN ORGANIZATION OF CRITHIDIA-FASCICULATA KINETOPLAST DNA-REPLICATION PROTEINS DURING THE CELL-CYCLE", The Journal of cell biology, 143(4), 1998, pp. 911-919

Abstract

Kinetoplast DNA (kDNA), the mitochondrial DNA in kinetoplastids, is anetwork containing several thousand topologically interlocked minicircles. We investigated cell cycle-dependent changes in the localizationof kDNA replication enzymes by combining immunofluorescence with either hydroxyurea synchronization or incorporation of fluorescein-dUTP into the endogenous gaps of newly replicated minicircles. We found that while both topoisomerase II and DNA polymerase beta colocalize in two antipodal sites flanking the kDNA during replication, they behave differently at other times. Polymerase beta is not detected by immunofluorescence either during cell division or G1, but is abruptly detected inthe antipodal sites at the onset of kDNA replication. In contrast, topoisomerase II is localized to sites at the network edge at all cell cycle stages; usually it is found in two antipodal sites, but during cytokinesis each postscission daughter network is associated with only asingle site. During the subsequent G1, topoisomerase accumulates in asecond localization site, forming the characteristic antipodal pattern. These data suggest that these sites at the network periphery are permanent components of the mitochondrial architecture that function in kDNA replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 09:24:58