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Titolo:
AUTORADIOGRAPHIC DISCRIMINATION OF MELANOCORTIN RECEPTORS INDICATES THAT THE MC3 SUBTYPE DOMINATES IN THE MEDIAL RAT-BRAIN
Autore:
LINDBLOM J; SCHIOTH HB; LARSSON A; WIKBERG JES; BERGSTROM L;
Indirizzi:
UPPSALA UNIV,BMC,DEPT PHARMACEUT BIOSCI,DIV PHARMACOL,BOX 591 S-75124UPPSALA SWEDEN
Titolo Testata:
Brain research
fascicolo: 1-2, volume: 810, anno: 1998,
pagine: 161 - 171
SICI:
0006-8993(1998)810:1-2<161:ADOMRI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOCYTE-STIMULATING HORMONE; MOLECULAR-CLONING; ALPHA-MSH; RADIOLIGAND BINDING; IN-SITU; EXPRESSION; PEPTIDES; LOCALIZATION; MELANOTROPIN; INHIBITION;
Keywords:
AUTORADIOGRAPHY; MELANOCORTIN RECEPTOR; GAMMA-1-MSH; HS014; RAT BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
J. Lindblom et al., "AUTORADIOGRAPHIC DISCRIMINATION OF MELANOCORTIN RECEPTORS INDICATES THAT THE MC3 SUBTYPE DOMINATES IN THE MEDIAL RAT-BRAIN", Brain research, 810(1-2), 1998, pp. 161-171

Abstract

In the present study, we developed an autoradiographic method to visualize the distribution of melanocortin (MC) receptors 3 and 4 in sagittal sections of the rat brain. The method takes advantage of the MC3 and MC4 receptor selective compounds, gamma 1-MSH and HS014. First, we characterized the binding of gamma 1-MSH, HS014 and the radioligand [I-125]NDP-MSH to the rat MC3 and MC4 receptors expressed in COS cells. [I-125]NDP-MSH was found to be non-selective, whereas yl-MSH showed a 40-fold preference for the rat MC3 receptor, and HS014 an over 300-fold preference to the rat MC4 receptor. Second, to discriminate between the MC3 and MC4 receptors in rat brain sections, the sections were incubated with [I-125]NDP-MSH in the presence of graded concentrations ofthe MC3 selective ligand, gamma 1-MSH, or the MC4 selective ligand, HS014. From the autoradiograms thus made, competition curves of gamma 1-MSH and HS014 could be constructed for different regions of the rat brain. Our results indicate that in the nucleus accumbens shell, the medial preoptic area, and the ventromedial nucleus of the hypothalamus, there is a clear dominance of the MC3 receptor, whereas in the lateralseptum and the olfactory tubercle, there seem to be present both MC3 and MC4 receptors, although the MC3 receptor may still be the dominating subtype. In the optic layer of the superior colliculus, our data indicate a more abundant expression of the MC4 receptor. in the ventral tegmental area, there might be an additional MSH-peptide binding site of unknown origin. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 06:38:48