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Titolo:
GENE ACTIVATION BY CYTOPLASMIC ACIDIFICATION IN SUSPENSION-CULTURED RICE CELLS IN RESPONSE TO THE POTENT ELICITOR, N-ACETYLCHITOHEPTAOSE
Autore:
HE DY; YAZAKI Y; NISHIZAWA Y; TAKAI R; YAMADA K; SAKANO K; SHIBUYA N; MINAMI E;
Indirizzi:
NATL INST AGROBIOL RESOURCES,DEPT BIOTECHNOL,LAB GLYCOBIOL,2-1-2 KANNONDAI TSUKUBA IBARAKI 3058602 JAPAN NATL INST AGROBIOL RESOURCES,DEPT BIOTECHNOL,LAB GLYCOBIOL TSUKUBA IBARAKI 3058602 JAPAN NATL INST AGROBIOL RESOURCES,DEPT PLANT PHYSIOL,LAB STRESS PHYSIOL TSUKUBA IBARAKI 3058602 JAPAN NATL INST AGROBIOL RESOURCES,DEPT BIOTECHNOL,LAB GENE ENGN TSUKUBA IBARAKI 3058602 JAPAN UNIV TSUKUBA,INST APPL BIOCHEM TSUKUBA IBARAKI 3058572 JAPAN
Titolo Testata:
Molecular plant-microbe interactions
fascicolo: 12, volume: 11, anno: 1998,
pagine: 1167 - 1174
SICI:
0894-0282(1998)11:12<1167:GABCAI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-AFFINITY BINDING; PROTEIN PHOSPHATASE INHIBITOR; PHENYLALANINE AMMONIA-LYASE; PLANT DEFENSE RESPONSES; ACETYLCHITOOLIGOSACCHARIDE ELICITOR; PLASMA-MEMBRANE; TOMATO CELLS; SIGNAL-TRANSDUCTION; INTRACELLULAR PH; PHYTOALEXIN PRODUCTION;
Keywords:
PROTEIN PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
D.Y. He et al., "GENE ACTIVATION BY CYTOPLASMIC ACIDIFICATION IN SUSPENSION-CULTURED RICE CELLS IN RESPONSE TO THE POTENT ELICITOR, N-ACETYLCHITOHEPTAOSE", Molecular plant-microbe interactions, 11(12), 1998, pp. 1167-1174

Abstract

N-Acetylchitoheptaose strongly induces a set of defense reactions in suspension-cultured rice cells including cytoplasmic acidification (K. Kuchitsu, Y. Yazaki, K. Sakano, and N. Shibuya, Plant Cell Physiol. 38:1012-1018, 1997) and the accumulation of mRNAs for two rapidly activated genes, EL2 and EL3 (E. Minami, K. Kuchitsu, D.-Y. He, H. Kouchi, N. Midoh, Y. Ohtsuki, and N. Shibuya, Plant Cell Physiol. 37:563-567, 1996), as well as phenylalanine ammonia-lyase (PAL), chitinase and beta-1,3-glucanase. Treatment of cells with propionic acid resulted in the accumulation of the mRNAs for EL2, EL3, and PAL in a manner similar to the accumulation induced by N-acetylchitoheptaose. Concomitantly, there was a rapid decrease in the cytoplasmic pH as detected with in vivo P-31-nuclear magnetic resonance (NMR) spectroscopy. Interestingly, K-252a, a potent inhibitor of Ser/Thr protein kinases, strongly inhibited gene induction by N-acetylchitoheptaose, but showed much less inhibition of gene induction caused by propionic acid. Calyculin A, a protein phosphatase inhibitor, induced mRNA accumulations for EL2, EL3 andPAL, with concomitant acidification of the cytoplasm. On the other hand, chitinase and P-glucanase mRNA levels did not change after addition of propionic acid or calyculin A. Treatment of the cells with propionic acid did not induce the production of reactive oxygen species. These results strongly suggest that cytoplasmic acidification at the early stage of elicitor action could be a key step in the signal transduction events leading to the expression of elicitor-responsive genes. A hypothetical model of elicitor signal pathway is proposed based on these results.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 11:54:49