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Titolo:
CHARACTERIZATION OF THE EGG VESICULAR COMPONENTS IN THE SEAWEED, FUCUS-SERRATUS L (FUCALES, PHAEOPHYTA), USING ENZYME-HISTOCHEMISTRY AND VITAL STAINING - THE SEARCH FOR A LYSOSOME-LIKE BODY
Autore:
HOLLAND RD; PITT D; MOORE MN; BROWNLEE C;
Indirizzi:
UNIV EXETER,DEPT SCI BIOL,WASHINGTON SINGER LABS,PERRY RD EXETER EX4 4QG DEVON ENGLAND PLYMOUTH MARINE LAB PLYMOUTH PL1 2PB DEVON ENGLAND MARINE BIOL ASSOC UNITED KINGDOM LAB PLYMOUTH PL1 2PB DEVON ENGLAND
Titolo Testata:
Histochemical Journal
fascicolo: 3, volume: 29, anno: 1997,
pagine: 239 - 248
SICI:
0018-2214(1997)29:3<239:COTEVC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
BROWN-ALGAE; CYTOCHEMICAL CHARACTERIZATION; ELECTRON-MICROSCOPE; PHENOLIC-COMPOUNDS; CORRELATED LIGHT; DIGESTIVE CELLS; LOCALIZATION; BODIES; POLYSACCHARIDES; FLUOROCHROMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
82
Recensione:
Indirizzi per estratti:
Citazione:
R.D. Holland et al., "CHARACTERIZATION OF THE EGG VESICULAR COMPONENTS IN THE SEAWEED, FUCUS-SERRATUS L (FUCALES, PHAEOPHYTA), USING ENZYME-HISTOCHEMISTRY AND VITAL STAINING - THE SEARCH FOR A LYSOSOME-LIKE BODY", Histochemical Journal, 29(3), 1997, pp. 239-248

Abstract

Fucus serratus eggs were examined for evidence of the existence of a lysosome-like body using enzyme histochemical and vital staining techniques. Simultaneous coupling ate-dye techniques for lysosomal acid phosphatase proved inappropriate owing to endogenous phenolic binding artefacts. The large number of alginate polysaccharide and polyphenolic egg vesicles interfered with vital staining techniques for lysosomes. Lysosomal esterase activity was detected in the abundant egg lipid bodies. The role of the egg lipid body as an equivalent lysosome-like bodyof higher plants, the spherosome, is discussed in relation to egg fertilization and early zygote development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 19:15:18