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Titolo:
POSITIONAL ISOMERS OF MONOPEGYLATED INTERFERON ALPHA-2A - ISOLATION, CHARACTERIZATION, AND BIOLOGICAL-ACTIVITY
Autore:
MONKARSH SP; MA YM; AGLIONE A; BAILON P; CIOLEK D; DEBARBIERI B; GRAVES MC; HOLLFELDER K; MICHEL H; PALLERONI A; PORTER JE; RUSSOMAN E; ROY S; PAN YCE;
Indirizzi:
HOFFMANN LA ROCHE INC,DEPT ONCOL,340 KINGSLAND ST NUTLEY NJ 07110 HOFFMANN LA ROCHE INC,DEPT ONCOL NUTLEY NJ 07110 HOFFMANN LA ROCHE INC,DEPT BIOPHARMACEUT NUTLEY NJ 07110 HOFFMANN LA ROCHE INC,DEPT ANALYT RES & DEV NUTLEY NJ 07110 ROCHE RES CTR WELWYN GARDEN CIT AL7 3AY HERTS ENGLAND
Titolo Testata:
Analytical biochemistry
fascicolo: 2, volume: 247, anno: 1997,
pagine: 434 - 440
SICI:
0003-2697(1997)247:2<434:PIOMIA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-CELLS; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
S.P. Monkarsh et al., "POSITIONAL ISOMERS OF MONOPEGYLATED INTERFERON ALPHA-2A - ISOLATION, CHARACTERIZATION, AND BIOLOGICAL-ACTIVITY", Analytical biochemistry, 247(2), 1997, pp. 434-440

Abstract

The success of recombinant interferon alpha in the clinic in part is limited by two properties of the protein: short serum half-life and immunogenicity. To improve these properties, interferon alpha-2a was conjugated with polyethylene glycol (PEG-5000). A homogeneous preparationof monopegylated interferon alpha-2a was subjected to vigorous analytical and activity characterization. A newly developed ampholyte-free chromatofocussing-like cation-exchange HPLC method utilizing a sulfopropyl resin was used to separate the monopegylated protein into 11 species. Peptide mapping, sequencing, and mass spectrometric analyses indicated that these species are positional isomers where each isomer represents a single polymer molecule conjugated to one specific lysine residue. No species with a modification at the amino terminus was observed. All 11 isomers show antiviral and antiproliferative activities in the same range as the parent monopegylated interferon alpha-2a. (C) 1997Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:28:31