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Titolo:
RECEPTOR RECOGNITION BY HISTIDINE 16 OF HUMAN EPIDERMAL GROWTH-FACTORVIA HYDROGEN-BOND DONOR ACCEPTOR INTERACTIONS/
Autore:
NANDAGOPAL K; TERZAGHIHOWE M; NIYOGI SK;
Indirizzi:
UNIV TENNESSEE,OAK RIDGE GRAD SCH BIOMED SCI,DIV LIFE SCI,OAK RIDGE NATL LAB,POB 2009 OAK RIDGE TN 37831 UNIV TENNESSEE,OAK RIDGE GRAD SCH BIOMED SCI,DIV LIFE SCI,OAK RIDGE NATL LAB OAK RIDGE TN 37831 PROT ENGN & MOL MUTAGENESIS PROGRAM OAK RIDGE TN 37831
Titolo Testata:
Journal of cellular biochemistry
fascicolo: 1, volume: 72, anno: 1999,
pagine: 16 - 24
SICI:
0730-2312(1999)72:1<16:RRBH1O>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-DIRECTED MUTAGENESIS; FACTOR EGF MUTANT; FACTOR-ALPHA; TGF-ALPHA; KINASE-ACTIVITY; BINDING; RESIDUES; EXPRESSION; AFFINITY; EXCHANGE;
Keywords:
CELL PROLIFERATION; LIGAND RECEPTOR INTERACTION; PROTEIN ENGINEERING; RATIONAL DESIGN; TYROSINE KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
K. Nandagopal et al., "RECEPTOR RECOGNITION BY HISTIDINE 16 OF HUMAN EPIDERMAL GROWTH-FACTORVIA HYDROGEN-BOND DONOR ACCEPTOR INTERACTIONS/", Journal of cellular biochemistry, 72(1), 1999, pp. 16-24

Abstract

Human epidermal growth factor (hEGF) and human transforming growth factor alpha (hTGF alpha) are prototypical of structurally related polypeptide mitogens which interact with the epidermal growth factor receptor (EGFR). Several determinants of receptor recognition that specify function have been proposed on the basis of structural criteria. This study evaluates the role of one such candidate, H16 of hEGF, by site-specific mutagenesis. When assayed for receptor tyrosine kinase stimulation using (Glu(4),Tyr(1))(n) as the exogenous substrate in vitro, the relative agonist activities of position 16 mutants range from 14-263% of wild-type hEGF. The rank order of potency was round to correlate with the relative receptor binding affinities of the mutants, which range from 7-272% of wild-type, as determined by radioreceptor competitionassays. The mitogenic activity of the H16 mutants is similar to that of wild-type hEGF as determined by clonogenic assays using rat tracheal epithelial cells. While the colony forming efficiencies do not reflect significant differences in growth rate or survival characteristics in the presence of the hEGF variants, it is reduced to 1.6% in controlcultures which lack EGF in the medium. The results show that H16 of hEGF, although not essential for mitogenic activity, optimizes receptorrecognition by hydrogen-bond donor/acceptor interactions and may share this feature with H18 of hTGF alpha. J. Cell. Biochem, 72:16-24, 1999. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 21:17:17