Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PROTEIN-PHOSPHORYLATION IS ESSENTIAL FOR FORMATION OF MALE PRONUCLEUSIN BOVINE OOCYTES
Autore:
CHIAN RC; TAN SL; SIRARD MA;
Indirizzi:
ROYAL VICTORIA HOSP,DEPT OBSTET & GYNECOL,MCGILL REPROD CTR,687 PINE AVE W MONTREAL PQ H3A 1A1 CANADA MCGILL UNIV,DEPT OBSTET & GYNECOL,MCGILL REPROD CTR MONTREAL PQ H3A 2T5 CANADA UNIV LAVAL,DEPT ANIM SCI QUEBEC CITY PQ CANADA
Titolo Testata:
Molecular reproduction and development
fascicolo: 1, volume: 52, anno: 1999,
pagine: 43 - 49
SICI:
1040-452X(1999)52:1<43:PIEFFO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
OKADAIC ACID; MOUSE OOCYTES; CELL-CYCLE; 6-DIMETHYLAMINOPURINE 6-DMAP; TYROSINE PHOSPHORYLATION; MEIOTIC MATURATION; GOLDEN-HAMSTER; CHROMATIN DECONDENSATION; INVITRO FERTILIZATION; MEIOSIS REINITIATION;
Keywords:
PHOSPHORYLATION; MALE PRONUCLEUS; BOVINE ZYGOTES; 6-DMAP; OA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
R.C. Chian et al., "PROTEIN-PHOSPHORYLATION IS ESSENTIAL FOR FORMATION OF MALE PRONUCLEUSIN BOVINE OOCYTES", Molecular reproduction and development, 52(1), 1999, pp. 43-49

Abstract

This study examined the association between the morphological and protein phosphorylation events following sperm penetration of in vitro matured and in vitro fertilized bovine oocytes. Oocytes were labeled with [P-32]-orthophosphate at 3 hr intervals from 3 to 18 hr of followinginsemination. The phosphorylation of protein complexes of 23 kDa and 18 kDa specifically increased with the formation of male and female pronuclei. In addition, oocytes were treated with 6-Dimethylaminopurine (6-DMAP) or Okadaic acid (OA) at 0, 3, 6, and 9 hr respectively following insemination. Although the formation of female pronucleus was not affected by 6-DMAP, the male pronuclear formation was completely inhibited by the presence of 6-DMAP at 0 and 3 hr of post insemination. Theformation of both pronuclei was inhibited by the presence of OA at any time following insemination. These results suggest that the male pronuclear formation is associated with protein phosphorylation and that the formation of the male and the female pronuclei may involve different factors in bovine zygotes since they respond differently to the kinase modulations. Mol. Reprod. Dev. 52:43-49, 1999. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:46:37