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Titolo:
NEUTROPHILS STIMULATED WITH A VARIETY OF CHEMOATTRACTANTS EXHIBIT RAPID ACTIVATION OF P21-ACTIVATED KINASES (PAKS) - SEPARATE SIGNALS ARE REQUIRED FOR ACTIVATION AND INACTIVATION OF PAKS
Autore:
HUANG RY; LIAN JP; ROBINSON D; BADWEY JA;
Indirizzi:
BOSTON BIOMED RES INST,20 STANIFORD ST BOSTON MA 02114 BOSTON BIOMED RES INST BOSTON MA 02114 MASSACHUSETTS GEN HOSP,ARTHRITIS UNIT BOSTON MA 02114 HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOL PHARMACOL BOSTON MA 02114
Titolo Testata:
Molecular and cellular biology (Print)
fascicolo: 12, volume: 18, anno: 1998,
pagine: 7130 - 7138
SICI:
0270-7306(1998)18:12<7130:NSWAVO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE; POLYMORPHONUCLEAR LEUKOCYTES; TYROSINE PHOSPHORYLATION; CALCIUM MOBILIZATION; CHEMOTACTIC FACTORS; CYTOSKELETAL ACTIN; ADAPTER PROTEIN; L-SELECTIN; MYOSIN-I; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
R.Y. Huang et al., "NEUTROPHILS STIMULATED WITH A VARIETY OF CHEMOATTRACTANTS EXHIBIT RAPID ACTIVATION OF P21-ACTIVATED KINASES (PAKS) - SEPARATE SIGNALS ARE REQUIRED FOR ACTIVATION AND INACTIVATION OF PAKS", Molecular and cellular biology (Print), 18(12), 1998, pp. 7130-7138

Abstract

Activation of the p21-activated protein kinases (Paks) was compared in neutrophils stimulated with a wide variety of agonists that bind to receptors coupled to heterotrimeric G proteins. Neutrophils stimulatedwith sulfatide, a ligand for the L-selectin receptor, or the chemoattractant fMet-Leu-Phe (fMLP), platelet-activating factor, leukotriene B-4, interleukin-8, or the chemokine RANTES exhibited a rapid and transient activation of the 63- and 69-kDa Paks. These kinases exhibited maximal activation,vith each of these agonists within 15 s followed by significant inactivation at 3 min. In contrast, neutrophils treated with the chemoattractant and anaphylatoxin C5a exhibited a prolonged activation (>15 min) of these Paks even though the receptor for this ligand may activate the same overall population of complex G proteins as the fMLP receptor, Addition of fMLP to neutrophils already stimulated with C5a resulted in the inactivation of the 63- and 69-kDa Paks. Optimal activation of Paks could be observed at concentrations of these agonists that elicited only shape changes and chemotaxis in neutrophils. While all of the agonists listed above triggered quantitatively similaractivation of the 63- and 69-kDa Paks, fMLP was far superior to the other stimuli in triggering activation of the c-Jun N-terminal kinase (JNK) and the p38 mitogen-activated protein kinase (MAPK). These data indicate that separate signals are required for activation and inactivation of Paks and that, in contrast to other cell types, activated Pak does not trigger activation of JNK or p38-MAPK in neutrophils. These results are consistent with the recent hypothesis that G-protein-coupled receptors may initiate signals independent of those transmitted by the alpha and beta gamma subunits of complex G proteins.

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Documento generato il 15/01/21 alle ore 23:18:20