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Titolo:
PARTIAL CHARACTERIZATION OF THE CALCIUM-RELEASING ACTIVITY OF PORCINESPERM CYTOSOLIC EXTRACTS
Autore:
WU H; HE CL; JEHN B; BLACK SJ; FISSORE RA;
Indirizzi:
UNIV MASSACHUSETTS,DEPT VET & ANIM SCI,PAIGE LABS AMHERST MA 01003 UNIV MASSACHUSETTS,DEPT VET & ANIM SCI,PAIGE LABS AMHERST MA 01003 UNIV MASSACHUSETTS,MOL & CELLULAR BIOL PROGRAM AMHERST MA 01003 UNIV ERLANGEN NURNBERG,DEPT IMMUNOL D-8520 ERLANGEN GERMANY
Titolo Testata:
Developmental biology (Print)
fascicolo: 2, volume: 203, anno: 1998,
pagine: 369 - 381
SICI:
0012-1606(1998)203:2<369:PCOTCA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN OOCYTE ACTIVATION; MOUSE OOCYTES; INTRACYTOPLASMIC INJECTION; MAMMALIAN OOCYTES; EGG ACTIVATION; CA2+ RELEASE; HAMSTER EGGS; FERTILIZATION; OSCILLATIONS; TRIGGERS;
Keywords:
SPERM FACTOR; [CA2+](I) OSCILLATIONS; MAMMALIAN EGGS; OSCILLIN; EGG ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
H. Wu et al., "PARTIAL CHARACTERIZATION OF THE CALCIUM-RELEASING ACTIVITY OF PORCINESPERM CYTOSOLIC EXTRACTS", Developmental biology (Print), 203(2), 1998, pp. 369-381

Abstract

Injection of sperm cytosolic extracts into mammalian eggs has been shown to elicit intracellular calcium ([Ca2+](i)) oscillations that are similar in amplitude, duration, and frequency to those observed following fertilization. Thus, to characterize the Ca2+-release component(s)in porcine sperm cytosolic extracts, a combination of fractionation techniques was used. The fraction with Ca2+ releasing activity was precipitated by 50% saturating solutions of ammonium sulfate and Western blot analysis showed that the pellets contained glucosamine-6-phosphatedeaminase (gpd)/oscillin, a protein which has been suggested to be the sperm's active component. Single and double isoelectrofocusing (IEF)of porcine sperm extracts generated fractions with different Ca2+-releasing activities. Fractions with maximal Ca2+-releasing activity did not contain material that was immunoreactive with antibodies against gpd/oscillin; adjacent fractions containing gpd/oscillin had no Ca2+-releasing activity. These findings were confirmed by IEF coupled with size exclusion chromatography on Superose 12 and with hydroxyapatite chromatography. These procedures predict an isoelectric point of our active component of 6.5-7.0 and a relative molecular weight ranging from 29 to 68 kDa. In summary, the data show that the Ca2+ release-inducing component(s) of porcine sperm extracts can be fractionated and that gpd/oscillin is not the pig sperm Ca2+ oscillogen. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 20:37:59