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Titolo:
CELLULAR COMPOSITION AND VIABILITY OF DEMI-EMBRYOS AND QUARTER-EMBRYOS MADE FROM BISECTED BOVINE MORULAE AND BLASTOCYSTS PRODUCED IN-VITRO
Autore:
RHO GJ; JOHNSON WH; BETTERIDGE KJ;
Indirizzi:
UNIV GUELPH,DEPT BIOMED SCI GUELPH ON N1G 2W1 CANADA UNIV GUELPH,DEPT BIOMED SCI GUELPH ON N1G 2W1 CANADA UNIV GUELPH,DEPT POPULAT MED GUELPH ON N1G 2W1 CANADA
Titolo Testata:
Theriogenology
fascicolo: 6, volume: 50, anno: 1998,
pagine: 885 - 895
SICI:
0093-691X(1998)50:6<885:CCAVOD>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOZYGOTIC TWINS; ZONA-PELLUCIDA; IN-VITRO; TROPHOBLASTIC CELLS; DEMIEMBRYOS; SURVIVAL; STAGE; MASS; INVITRO; CALVES;
Keywords:
BOVINE; IVP EMBRYOS; BISECTION; QUARTER-EMBRYOS; EMBRYO VIABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
G.J. Rho et al., "CELLULAR COMPOSITION AND VIABILITY OF DEMI-EMBRYOS AND QUARTER-EMBRYOS MADE FROM BISECTED BOVINE MORULAE AND BLASTOCYSTS PRODUCED IN-VITRO", Theriogenology, 50(6), 1998, pp. 885-895

Abstract

The cellular composition and viability of intact, IVP embryos were compared with those of demi- and quarter-embryos produced by bisection of IVP morulae and blastocysts. Embryos were produced by established techniques from oocytes harvested from slaughterhouse ovaries. In Experiment 1, morulae at Day 6 or blastocysts at Day 7 were bisected on an inverted microscope using a microsurgical steel blade. Demi-embryos were then cultured without a zona pellucida until Day 8, when they were morphologically assessed for quality (viability). A higher proportion of demi-embryos made from blastocysts than from morulae were classifiedas viable (381/420, 91% vs 164/267, 61%; P<0.001). In Experiment 2, only Day 7 blastocysts were bisected, and some of the resulting demi-embryos were bisected a second time 24 h later to produce quarter-embryos. The remaining demi-embryos, the quarter-embryos, and control intactembryos were cultured until Day 9, at which time they were assessed for quality and subjected to immunosurgery and differential staining tocount inner cell mass (ICM) and trophectoderm cells. A higher proportion of demi-embryos than quarter-embryos was classified as viable (408/459, 89% vs 223/319, 70%, respectively; P<0.001). Total cell numbers decreased with successive bisections, but the proportion of surviving cells found in the ICM was significantly (P<0.05) higher in the best quality demi- and quarter-embryos (35 and 32%, respectively) than in the controls (22%). Transfer of all 12 quarter-embryos derived from 3 blastocysts, in pairs, into 6 recipient heifers resulted in 2 pregnancies, each with a single viable fetus at 90 d of gestation. The fetuses originated from 2 different blastocysts. The results suggest that bisection of intact IVP embryos into demi-embryos and bisection of those into quarter-embryos can increase the number of transferable embryos by as much as 178 and 235%, respectively. (C) 1998 by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:17:27